Investigating the mechanism of phosphate aquisition by Pseudomonas aeruginosa

铜绿假单胞菌获取磷酸盐的机制研究

• 批准号: 401975-2011
• 负责人: Moraes, Trevor
• 金额: $ 2.99万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=529375
• 关键词: Investigating; mechanism; phosphate; aquisition; Pseudomonas

Phosphate is an essential nutrient that is found in lipids, nucleic acids, proteins and sugars and is also key to many biological reactions that require ATP and/or signaling pathways that exploit protein phosphorylation. Thus, bacteria utilize a global regulatory circuit, the Pho regulon, to manage intracellular bacterial phosphate homeostasis. The Pho regulon regulates the expression of key proteins involved in the acquisition of phosphate, including integral membrane proteins that play an important role in the transport of phosphate across lipid bilayers and into the cell. These membrane proteins also act as sensory proteins that interact with phosphate binding proteins to detect the levels of extracellular phosphate and in turn regulate their own expression through the Pho regulon. The Pho regulon pathway also controls many other gene networks including the expression and secretion of virulence factors in response to phosphate levels, thus this phosphate acquisition pathway in bacteria is extremely important.

磷酸盐是一种必需的养分，它在脂质，核酸，蛋白质和糖中发现，也是许多需要ATP和/或信号传导途径的生物反应的关键，这些途径可以利用蛋白质磷酸化。 因此，细菌利用全球调节回路PHO调节量来管理细胞内细菌磷酸盐稳态。 PHO调节量调节与磷酸盐采集有关的关键蛋白的表达，包括整体膜蛋白，这些膜蛋白在磷酸盐跨脂质双层和细胞中发挥重要作用。 这些膜蛋白还充当感官蛋白，与磷酸盐结合蛋白相互作用以检测细胞外磷酸盐的水平，进而通过Pho congulon调节其自身的表达。 PHO调节途径还控制着许多其他基因网络，包括响应磷酸盐水平的毒力因子的表达和分泌，因此细菌中这种磷酸盐采集途径非常重要。