Investigating the mechanism of phosphate aquisition by Pseudomonas aeruginosa

铜绿假单胞菌获取磷酸盐的机制研究

• 批准号: 401975-2011
• 负责人: Moraes, Trevor
• 金额: $ 2.99万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=529375
• 关键词: Investigating; mechanism; phosphate; aquisition; Pseudomonas

Phosphate is an essential nutrient that is found in lipids, nucleic acids, proteins and sugars and is also key to many biological reactions that require ATP and/or signaling pathways that exploit protein phosphorylation. Thus, bacteria utilize a global regulatory circuit, the Pho regulon, to manage intracellular bacterial phosphate homeostasis. The Pho regulon regulates the expression of key proteins involved in the acquisition of phosphate, including integral membrane proteins that play an important role in the transport of phosphate across lipid bilayers and into the cell. These membrane proteins also act as sensory proteins that interact with phosphate binding proteins to detect the levels of extracellular phosphate and in turn regulate their own expression through the Pho regulon. The Pho regulon pathway also controls many other gene networks including the expression and secretion of virulence factors in response to phosphate levels, thus this phosphate acquisition pathway in bacteria is extremely important.

磷酸盐是在脂质、核酸、蛋白质和糖中发现的必需营养素，并且也是许多需要ATP和/或利用蛋白质磷酸化的信号通路的生物反应的关键。 因此，细菌利用全局调节回路Pho调节子来管理细胞内细菌磷酸盐稳态。 Pho调节子调节参与磷酸盐获得的关键蛋白的表达，包括在磷酸盐跨脂质双层转运并进入细胞中发挥重要作用的整合膜蛋白。 这些膜蛋白还充当感觉蛋白，其与磷酸盐结合蛋白相互作用以检测细胞外磷酸盐的水平，并进而通过Pho调节子调节其自身的表达。 Pho调节子途径还控制许多其他基因网络，包括响应磷酸盐水平的毒力因子的表达和分泌，因此这种磷酸盐获取途径在细菌中极其重要。