Dissecting small RNA pathways using comparative genetics and genomics in C. elegans and C. briggsae

使用比较遗传学和基因组学剖析线虫和布里格斯线虫的小 RNA 通路

• 批准号: 418683-2012
• 负责人: Claycomb, Julie
• 金额: $ 2.19万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=531317
• 关键词: Dissecting; small; RNA; pathways; using

RNA interference (RNAi) is a cellular process whereby the expression of a specific gene is silenced by the action of an enzymatic complex composed of a small RNA molecule acting with an Argonaute protein. These small RNA molecules provide sequence-specific gene targeting activity to the Argonaute, which is the catalyst of silencing. RNAi has been the topic of much interest over the past decade, during which time it has become a nearly ubiquitous laboratory tool, revolutionizing studies in some research systems that were previously genetically intractable. RNAi also holds great therapeutic potential; imagine being able to turn off a specific gene that contributed to cancer progression at will, thus halting oncogenesis.

RNA干扰（RNAi）是一种细胞过程，通过与Argonaute蛋白作用的小RNA分子组成的酶复合物的作用使特定基因的表达沉默。这些小RNA分子为Argonaute提供序列特异性基因靶向活性，这是沉默的催化剂。在过去的十年里，RNAi一直是人们非常感兴趣的话题，在这段时间里，它已经成为一种几乎无处不在的实验室工具，在一些以前在遗传上难以解决的研究系统中进行了革命性的研究。RNAi还具有巨大的治疗潜力；想象一下，可以随意关闭导致癌症进展的特定基因，从而阻止肿瘤的发生。