Novel tools for disrupting gene function in the developing chicken embryo

破坏发育中鸡胚胎基因功能的新工具

• 批准号: 431403-2012
• 负责人: Bendall, Andrew
• 金额: $ 0.98万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=532987
• 关键词: Novel; tools; disrupting; gene; function

The chicken is an economically important food animal for humans and the chicken embryo has long been an important model system for developmental biologists. Our overall goal is to understand the regulatory genetic pathways that control the growth and development of the skeleton. This proposal is directed towards building novel vectors to better control our ability to manipulate gene function during chicken embryo development. In particular, since strategies to knock down gene function in the chick embryo in ovo are not yet widely used, we propose to construct and validate retroviral plasmids for tissue-specific delivery of small RNAs that disrupt key regulators of cartilage maturation in order to better understand their roles in skeletal development. Our initial focus will be on a family of transcriptional regulators called Dlx proteins in order to understand to what extent these genes are functionally redundant in the cartilage-derived skeleton.

鸡是人类重要的经济食用动物，鸡胚一直是发育生物学家研究的重要模型系统。我们的总体目标是了解控制骨骼生长和发育的调控遗传途径。该建议旨在构建新的载体，以更好地控制我们在鸡胚发育过程中操纵基因功能的能力。特别是，由于在鸡卵细胞胚胎中敲除基因功能的策略尚未广泛应用，我们建议构建并验证逆转录病毒质粒，用于组织特异性递送破坏软骨成熟关键调节因子的小rna，以便更好地了解它们在骨骼发育中的作用。我们最初的重点将放在一个名为Dlx蛋白的转录调节因子家族上，以了解这些基因在软骨衍生骨骼中的功能冗余程度。