Identification of Giardia species from water samples by PCR and genotyping

通过 PCR 和基因分型鉴定水样中的贾第虫属

• 批准号: 446456-2012
• 负责人: MacGregor, Lorne
• 金额: $ 1.5万
• 依托单位: Lethbridge Community College
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 1
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=536333
• 关键词: Identification; Giardia; species; water; samples

Lethbridge College has partnered with Hyperion Research Ltd (Hyperion) to develop a rapid genotyping method for characterization of positive Giardia water samples. In this project a simple molecular diagnostic laboratory method will be developed using nested polymerase chain reactions (PCR), DNA sequencing and pyrosequencing techniques to differentiate human pathogenic Giardia strains from non-pathogenic strains isolated from Giardia positive water samples. These tools will give Hyperion Research Ltd. the ability to inform their customers of the public and human health risks of a positive Giardia test from a water sample. This project will also highlight Lethbridge College as a center for biotechnology innovation in Canada. Lethbridge College will also be able to use the techniques developed through this project to attract new

莱斯布里奇学院已与研究有限公司合作，开发一种快速基因分型方法，用于表征阳性贾第虫水样。本项目将利用巢式聚合酶链反应（PCR）、DNA测序和焦磷酸测序技术开发一种简单的分子诊断实验室方法，以区分人类致病性贾第虫菌株和从贾第虫阳性水样中分离的非致病性菌株。这些工具将使ESPRISE Research Ltd.能够告知其客户水样中贾第虫阳性检测对公众和人类健康的风险。该项目还将突出莱斯布里奇学院作为加拿大生物技术创新中心。莱斯布里奇学院也将能够使用通过这个项目开发的技术，以吸引新的