Identification of Giardia species from water samples by PCR and genotyping

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Lethbridge College has partnered with Hyperion Research Ltd (Hyperion) to develop a rapid genotyping method for characterization of positive Giardia water samples. In this project a simple molecular diagnostic laboratory method will be developed using nested polymerase chain reactions (PCR), DNA sequencing and pyrosequencing techniques to differentiate human pathogenic Giardia strains from non-pathogenic strains isolated from Giardia positive water samples. These tools will give Hyperion Research Ltd. the ability to inform their customers of the public and human health risks of a positive Giardia test from a water sample. This project will also highlight Lethbridge College as a center for biotechnology innovation in Canada. Lethbridge College will also be able to use the techniques developed through this project to attract new