Droplet digital PCR system for new applications in physiology, developmental biology, biomolecular interactions and natural product discovery

液滴数字 PCR 系统在生理学、发育生物学、生物分子相互作用和天然产物发现方面的新应用

• 批准号: 458621-2014
• 负责人: Trudeau, Vance
• 金额: $ 6.71万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=541987
• 关键词: Droplet; digital; PCR; system; new

Funds are requested for the purchase of new state-of-the art system that can perform the polymerase chain-reaction to amplify DNA for quantification. It is called droplet digital PCR, and is the most sensitive and accurate way to determine the levels of gene expression in tissues and cells. Many members of the Centre for Advanced Research in Environmental Genomics (CAREG) at the University of Ottawa study gene expression in different tissues or following treatments (e.g. exposure to environmental contaminants, hormones). As a group of 7 major users, we will use the machine to research an array of topics including brain function, hormones, bacterial natural products, beneficial fungi, and phytomedicines. Thus, the purpose of the present application is to fund a digital PCR system for the use of the research teams in CAREG and to train new graduate and undergraduate students in cutting-edge gene expression analysis. Not only will their research be at the cutting-edge, their training program will be enhanced, making them more competitive when seeking employment.

需要资金购买新的最先进的系统，该系统可以进行聚合酶链式反应，扩增DNA以进行量化。它被称为液滴数字PCR，是确定组织和细胞中基因表达水平的最灵敏和最准确的方法。渥太华大学环境基因组学高级研究中心（CAREG）的许多成员研究不同组织或治疗后（例如暴露于环境污染物，激素）的基因表达。作为一个由7个主要用户组成的小组，我们将使用该机器研究一系列主题，包括大脑功能、激素、细菌天然产物、有益真菌和植物药。因此，本申请的目的是为CAREG研究团队使用的数字PCR系统提供资金，并在尖端基因表达分析方面培训新的研究生和本科生。 不仅他们的研究将处于前沿，他们的培训计划将得到加强，使他们在求职时更具竞争力。