Surface Electrochemistry of DNA Conformational Switches

DNA 构象开关的表面电化学

• 批准号: 239068-2013
• 负责人: Yu, Hogan
• 金额: $ 5.03万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=547189
• 关键词: Surface; Electrochemistry; DNA; Conformational; Switches

The main goal of the proposed research is to obtain a thorough knowledge of the surface electrochemistry of functional deoxyribonucleic acids (DNA). It is essential to understand the nature of DNA which plays a pivotal role in biology as the carrier of genetic information. Besides typical single strands and double helices, complex DNA nanostructures (so-called DNA conformational switches) can be constructed to have desired functions or reactivities. Using standard biochemical assays, perfect double helices and properly designed DNA switches in solution have been found to efficiently mediate charge flow. However, this is not the consensus mechanism when they are bound to an electrode surface; in that case both DNA-mediated and direct electron transfer are supported by experimental observations.

拟议的研究的主要目标是获得功能脱氧核糖核酸（DNA）的表面电化学的透彻的知识。DNA作为遗传信息的载体，在生物学中起着举足轻重的作用，了解DNA的性质至关重要。除了典型的单链和双螺旋，复杂的DNA纳米结构（所谓的DNA构象开关）可以被构建为具有所需的功能或反应性。使用标准的生物化学测定，已经发现溶液中完美的双螺旋和适当设计的DNA开关有效地介导电荷流动。然而，当它们结合到电极表面时，这不是共识机制;在这种情况下，DNA介导的和直接的电子转移都得到了实验观察的支持。