Systematic identification of receptors required for nervous system development in C. elegans

系统鉴定线虫神经系统发育所需的受体

• 批准号: 312498-2012
• 负责人: Hutter, Harald
• 金额: $ 2.04万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=548767
• 关键词: Systematic; identification; receptors; required; nervous

During the development of the brain neurons migrate to their proper locations and send out thin processes (axons and dendrites). Axons grow sometimes over long distances into target regions where they connect to other neurons forming neuronal networks. We are studying the molecular mechanism of neuronal migration and axonal pathfinding. Tools and resources available in C. elegans allow large-scale screening strategies to identify genes controlling developmental processes. In this proposal we plan to screen systematically putative receptors for a role in neuronal migration processes. We established a list of predominantly uncharacterized transmembrane proteins (possibly novel receptors), which are expressed in neurons during embryonic development. We plan to systematically screen available mutants in those genes to identify potentially novel receptors required for neuronal development. We anticipate that this will uncover a number of genes, which are important for the migration of neurons or axons towards their targets. The strong evolutionary conservation of many genes found in C. elegans ensures that our findings will be of general importance for the understanding of migration processes during brain development also in vertebrates including humans.

在大脑发育过程中，神经元迁移到适当的位置，发出细小的突起(轴突和树突)。轴突有时会在很长的距离内生长到目标区域，在那里它们与其他神经元连接，形成神经元网络。我们正在研究神经元迁移和轴突寻路的分子机制。线虫可用的工具和资源使大规模筛选战略能够识别控制发育过程的基因。在这项提议中，我们计划系统地筛选在神经元迁移过程中扮演角色的假定受体。我们建立了一份主要未确定特征的跨膜蛋白(可能是新的受体)的清单，这些蛋白在胚胎发育期间在神经元中表达。我们计划系统地筛选这些基因中的可用突变体，以确定神经元发育所需的潜在新受体。我们预计，这将发现一些基因，这些基因对于神经元或轴突向其目标迁移非常重要。在线虫中发现的许多基因在进化上具有很强的保守性，这确保了我们的发现将对理解包括人类在内的脊椎动物大脑发育过程中的迁移过程具有普遍重要性。