Characterization of RND efflux pumps of acinetobacter baumannii

鲍曼不动杆菌 RND 外排泵的表征

• 批准号: 355806-2010
• 负责人: Kumar, Ayush
• 金额: $ 1.97万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=549832
• 关键词: Characterization; RND; efflux; pumps; acinetobacter

Acinetobacter baumannii is a rod-shaped Gram-negative bacterium that commonly inhabits soil. A. baumannii is highly resistant to various antimicrobials, and multidrug clinical isolates have been isolated all over the world including in Canada. Our hypothesis is that efflux pumps belonging to the Resistance-Nodulation-cell Division (RND) family aid in the survival of A. baumannii in the presence of harmful compounds (including antimicrobials, heavy metals, detergents, and dyes), and that characterization of these efflux pumps will contribute to an understanding of the survival mechanisms employed by this organism. Using powerful molecular and genetic tools, this program will characterize three RND complex-encoding operons,

鲍曼不动杆菌是一种杆状革兰氏阴性细菌，通常栖息在土壤中。A.鲍曼不动杆菌对各种抗微生物剂具有高度耐药性，并且在包括加拿大在内的世界各地已经分离出多药临床分离株。我们的假设是，属于抵抗-结瘤-细胞分裂（RND）家族的外排泵有助于A。在有害化合物（包括抗菌剂、重金属、洗涤剂和染料）的存在下对鲍曼不动杆菌的作用，并且这些外排泵的表征将有助于理解该生物体所采用的存活机制。使用强大的分子和遗传工具，该计划将表征三个RND复合编码操纵子，