A special group of RNA elements in splicing control

剪接控制中的一组特殊 RNA 元件

• 批准号: 385807-2010
• 负责人: Xie, Jiuyong
• 金额: $ 2.4万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=550268
• 关键词: special; group; RNA; elements; splicing

Alternative splicing is an common step in the control of gene expression in higher animal species, determining the number of proteins and their variations to be produced and playing critical roles in almost every aspect of animal life from cell growth/death, differentiation to development and physiology; however, the molecular bases of the many control elements at various RNA locations and their differences among species during evolution are still not clear. Our past work and current data identified a group of elements at an unusual location that, for the splicing machinery to function optimally, often does not have such regulatory elements. We hypothesize that this specially localized group of RNA elements are common in the genome to control splicing. In this proposal, we will use two of such elements "G tract" and "A-rich element" to test the hypothesis, using mutagenesis combined with RNA splicing assays as we have done previously. Answers to this question will provide a molecular basis for the function of this group of specially localized RNA elements, insights into their evolutionary evolvement and improve the prediction of RNA elements/alternative exons in the genomes in general.

选择性剪接是高等动物基因表达调控中的一个常见步骤，它决定了要产生的蛋白质的数量及其变异，在动物生命的几乎所有方面都发挥着关键作用，从细胞的生长/死亡、分化到发育和生理，然而，不同RNA位置上的许多控制元件的分子基础及其在进化过程中的物种差异仍然不清楚。我们过去的工作和目前的数据在一个不寻常的位置发现了一组元件，为了使剪接机器发挥最佳功能，这些元件通常不具有这样的调节元件。我们假设，这种特殊定位的RNA元件组在基因组中是常见的，以控制剪接。在这个建议中，我们将使用两个这样的元素“G轨迹”和“富A元素”来验证这一假设，使用突变结合RNA剪接分析，就像我们之前所做的那样。对这个问题的回答将为这组特殊定位的RNA元件的功能提供分子基础，洞察它们的进化进化，并从总体上改进对RNA元件/可选外显子的预测。