A special group of RNA elements in the evolution of alternative exons

替代外显子进化中的一组特殊 RNA 元件

• 批准号: RGPIN-2016-06004
• 负责人: Xie, Jiuyong
• 金额: $ 2.26万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=709791
• 关键词: special; group; RNA; elements; evolution

Studying how regulatory RNA elements evolved within splice sites gives a special angle for us to understand the regulation of alternative splicing as well as the origin of novel splice site/exons during evolution. In the work supported by my last NSERC grant, we have characterized the role of a special group of RNA elements that we call regulatory RNA elements between the polypyrimidine tract and 3'AG (REPA). Specifically, we have demonstrated the evolution, mechanism and functional impact of a particular group of REPA element, G tracts. In this proposal, we plan to (1) obtain a more complete distribution of REPA elements in the evolutionary tree; (2) elucidate the molecular mechanism of their regulation of splicing by taking advantage of the constrain that limits the presence of REPA in some locations and species; (3) estimate the contribution of REPA to the origin of novel alternative exons in the transcriptomes of different species. We hope this work will help us obtain a complete view of this special group of RNA elements among different species, its mechanisms of action and contribution to the origin of alternative exons and transcriptome complexity.

研究剪接位点内RNA调控元件的进化，为我们理解选择性剪接的调控以及新剪接位点/外显子在进化过程中的起源提供了一个特殊的视角。在我的最后一个NSERC资助的工作中，我们描述了一组特殊的RNA元件的作用，我们称之为多聚嘧啶道和3 'AG（REPA）之间的调节RNA元件。具体来说，我们已经证明了一组特定的REPA元件，G束的演变，机制和功能的影响。在这个计划中，我们计划（1）获得REPA元件在进化树中更完整的分布;（2）通过利用限制REPA在某些地点和物种中存在的限制来阐明它们调节剪接的分子机制;（3）估计REPA对不同物种转录组中新的替代外显子起源的贡献。我们希望这项工作将帮助我们获得一个完整的看法，这组特殊的RNA元件在不同的物种，其作用机制和贡献的替代外显子和转录组复杂性的起源。