Purchase of a small animal irradiator

购买小动物照射器

• 批准号: 472383-2015
• 负责人: Heinonen, Krista
• 金额: $ 10.93万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564736
• 关键词: Purchase; small; animal; irradiator

Biological irradiators, using either X-ray or radioactive sources, are often used in cell biology to introduce DNA damage and to stop cell proliferation. In addition to the use of irradiation as a direct method to study the impact of DNA damage to cell growth and/or the mechanisms associated with DNA repair, irradiation has other, widespread, potential applications: 1) total body irradiation will wipe out the animal's immune system, which can then be replaced by cells of different genetic composition to study the mechanisms of blood cell development or of the immune response; 2) targeted irradiation can be used to sterilize mice to study infertility and the generation and production of sperm; 3) irradiation of bacterial pathogens can serve to determine roles of specific components, growth conditions, or regulatory pathways in stress resistance that could contribute to survival on foods and virulence; and 4) irradiation of cells in culture can be used to reduce the number of confounding variables from experimental systems including more than one cell type. In brief, access to irradiation sources is essential for a wide variety of research programs in natural sciences.

生物辐照器，使用X射线或放射源，通常用于细胞生物学中，以引入DNA损伤并阻止细胞增殖。除了使用辐射作为研究DNA损伤对细胞生长的影响和/或与DNA修复相关的机制的直接方法之外，辐射还具有其他广泛的潜在应用：1）全身辐射将消灭动物的免疫系统，然后可以用不同遗传组成的细胞代替以研究血细胞发育或免疫应答的机制; 2）靶向照射可用于使小鼠绝育以研究不育和精子的产生和生产; 3）细菌病原体的照射可用于确定特定组分、生长条件或调节途径在应激抗性中的作用，这些应激抗性可有助于在食物上存活和毒力;和4）培养物中细胞的照射可用于减少来自包括一种以上细胞类型的实验系统的混杂变量的数目。简而言之，获得辐照源对于自然科学中的各种研究方案至关重要。