High-Capacity and Temperature-Controlled Shaker Incubators

大容量温控摇床

• 批准号: 472679-2015
• 负责人: Facchini, Peter
• 金额: $ 3.99万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564812
• 关键词: Capacity; Temperature; Controlled; Shaker; Incubators

Plant biology research at the University of Calgary has a long history of excellence and impact both in Canada and abroad. Current research is focused on (i) the biosynthesis of benzylisoquinoline alkaloids, such as the analgesics morphine and codeine, and the cough suppressant and anticancer drug noscapine, (ii) exploring the cellular signaling mechanisms that regulate compatible pollination events during plant reproduction and elucidating the complex interplay between compatibility and incompatibility pathways, (iii) the post-transcriptional regulation of gene expression in plant cells aimed at understanding the regulation of amino acid biosynthetic pathways, and the small RNA regulation of genes involves in nitrogen assimilation and storage, and (iv) the biochemistry of plant terpenoid metabolism targeting alternative production systems for natural rubber and other compounds. These research programs rely heavily on the growth of microorganisms to deploy advanced molecular biology technologies and develop novel metabolite engineering systems. Currently, there is a chronic shortage of variable-temperature shaker incubators in the Department of Biological Sciences. The four applicants oversee active research groups with a high, daily demand for the requested equipment. The Thermo Scientific MaxQ 8000 Incubated Stackable Shakers are designed to be stacked three high on the floor, and have the durability to run at top speed in that configuration. The three separate units provide flexible shaker speeds and incubation temperatures to meet the variable demands of highly qualified personnel in four highly active plant biology laboratories. Immediate availability of the equipment is essential to maintain a high level of training to at least six postdoctoral fellows, more than a dozen graduate students, and several undergraduate project students. The state-of-the-art equipment will also support the success in obtaining future research funding for all four applicants.

卡尔加里大学的植物生物学研究在加拿大和国外有着悠久的卓越历史和影响力。目前的研究重点是（i）苄基异喹啉生物碱的生物合成，例如止痛药吗啡和可待因，以及止咳药和抗癌药物诺斯卡品，（ii）探索调节植物繁殖过程中相容性授粉事件的细胞信号传导机制，并阐明相容性和不相容性途径之间复杂的相互作用，（iii） 植物细胞中基因表达的转录后调控旨在了解氨基酸生物合成途径的调控，以及涉及氮同化和储存的基因小RNA调控，以及（iv）针对天然橡胶和其他化合物的替代生产系统的植物萜类代谢的生物化学。这些研究项目在很大程度上依赖微生物的生长来部署先进的分子生物学技术并开发新型代谢工程系统。目前，生物科学系变温摇床培养箱长期短缺。四名申请人负责管理活跃的研究小组，这些小组每天对所要求的设备有很高的需求。 Thermo Scientific MaxQ 8000 孵化可堆叠摇床设计为在地板上堆叠三层高，并且具有在该配置下高速运行的耐用性。三个独立的单元提供灵活的摇床速度和孵化温度，以满足四个高度活跃的植物生物学实验室高素质人员的不同需求。设备的立即可用对于维持至少六名博士后研究员、十多名研究生和几名本科项目学生的高水平培训至关重要。最先进的设备还将支持所有四位申请人成功获得未来的研究经费。