Multifunctional microplate readers for assays of cellular activity and molecular quantification

用于细胞活性测定和分子定量的多功能酶标仪

• 批准号: 472798-2015
• 负责人: Cameron, Andrew
• 金额: $ 3.57万
• 依托单位: University of Regina
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564847
• 关键词: Multifunctional; microplate; readers; assays; cellular

It is well established that using an integrated approach is required to understand the complexity of biological organization. To achieve this, our six research groups study the signals and regulatory mechanisms involved in gene expression, the kinetics of protein activities, and cell physiology. We require laboratory equipment that can be shared between our collaborating research groups and used reliably for multiple different applications to advance our research programs and to continue to collaborate effectively with one another. Multifunctional microplate readers, one capable of culturing cells and measuring visible light absorbance, fluorescence, and luminescence (item 1) coupled with a microplate reader for measuring UV absorbance (item 2), will together provide a core platform for addressing many physiological, biochemical, and molecular questions. These indispensable tools will operate at maximum capacity for many years, supporting the research programs of six NSERC-funded researchers.

众所周知，使用综合方法来理解生物组织的复杂性是必要的。为了实现这一目标，我们的六个研究小组研究了基因表达、蛋白质活动动力学和细胞生理学中涉及的信号和调节机制。我们需要实验室设备可以在我们的合作研究小组之间共享，并可靠地用于多个不同的应用程序，以推进我们的研究计划，并继续有效地相互合作。多功能微孔板读取器，一个能够培养细胞和测量可见光吸光度，荧光和发光（项目1）加上一个微孔板读取器测量紫外线吸光度（项目2），将共同提供一个核心平台，解决许多生理，生化和分子问题。这些不可或缺的工具将以最大容量运行多年，支持六位nserc资助的研究人员的研究项目。