Multimode microplate reader with imaging for versatile analyses in immunology, microbiology, biochemistry and environmental toxicology

我们正在申请资金用于集成显微镜和成像的多模式微孔板阅读器。多模读卡器有许多用途，使其广泛使用，成为许多类型研究的重要工具。最近在功能上的进步已经大大扩展了测量和技术的范围，多模式微孔板读取器可以执行。由于这种广泛的通用性，在我们的研究小组中，多模式微孔板阅读器对于许多研究应用是必不可少的。例如，我们需要这台仪器来检查肠道微生物、饮食和运动对免疫系统的影响，用于开发使用siRNA的治疗性基因沉默策略，用于研究藻类在水生生态系统中的作用和环境污染物的生物转化的环境研究，用于开发用于五大湖环境影响监测的非致命性策略，并分析细胞相互作用，以检查骨和神经组织工程组织支架的生物相容性。目前，我们完全依赖于过时的读卡器，其零件不再可用，也不再支持维修。虽然我们自己已经完成了最近的修复，但很明显，微孔板读取器很快就会无法修复，这使我们无法进行我们日常进行的许多必要的分析和测量。该仪器对于保持我们的研究进展和支持安大略省理工大学高度协作的应用生物科学研究环境中的研究协同作用至关重要。与具有成像能力的当前模型多模式读取器一起工作，将为博士后培训生、研究生和本科生提供各种技术的市场技能，包括不断增加的生物测定和生物标志物定量。该仪器是分析各种细胞类型和生物体的理想选择，从细菌和藻类到鱼类胚胎和人类细胞，涵盖了我们高度合作的研究项目的研究重点。具有成像功能的多模式微孔板读卡器将被广泛使用，为具有市场技能的高竞争力HQP提供跨学科实践培训的前沿机会，以满足加拿大对生物技术、制药和农业食品工业以及环境监测相关专业知识日益增长的需求。

We are requesting funds for a multimode microplate reader with integrated microscopy and imaging. Multimode plate readers have numerous and versatile applications, making them widely used and essential tools for many types of research. Recent advances in functionality have greatly expanded the range of measures and techniques that multimode microplate readers can perform. Due to this broad versatility, a multimode microplate reader is essential for numerous research applications in our research groups. For example, we require this instrument to examine the impact of gut microbes, diet and exercise on the immune system, for development of therapeutic gene-silencing strategies using siRNA, for environmental research examining roles of algae in aquatic ecosystems and biotransformation of environmental contaminants, for development of non-lethal strategies for environmental effects monitoring in the Great Lakes, and for analysis of cellular interactions necessary to examine the biocompatibility of engineered tissue scaffolds for bone and nervous tissue. At present, we are completely reliant on an obsolete plate reader for which parts are no longer available and repair is no longer supported. While we have completed recent repairs ourselves, it is evident that the microplate reader will soon be beyond repair, leaving us unable to perform numerous essential assays and measures that we conduct on a daily basis. This instrument is essential to maintain our research progress and to support research synergies in the highly collaborative Applied Bioscience research environment at Ontario Tech. Working with a current model multimode reader with imaging ability will provide marketable skills to post-doctoral trainees and to graduate and undergraduate students in varied techniques including an ever-increasing array of bioassays and biomarker quantification. This instrument is ideal for analysis of a wide range of cell types and organisms, from bacteria and algae to fish embryos and human cells, encompassing the research foci of our highly collaborative research programs. The multimode microplate reader with imaging will be used intensively, providing cutting edge opportunities for interdisciplinary hands-on training of highly competitive HQP with marketable skills to meet growing needs in Canada for expertise relevant for biotechnology, pharmaceutical and agri-food industries and in environmental monitoring.