Using TRFLP sequencing to assess and monitor beneficial and detrimental soil nematodes in agricultural systems

使用 TRFLP 测序评估和监测农业系统中有益和有害的土壤线虫

• 批准号: 474772-2014
• 负责人: Lindo, Zoë
• 金额: $ 1.66万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=565086
• 关键词: Using; TRFLP; sequencing; assess; monitor

Understanding changes in free-living soil nematode assemblages under no-till and conventional tillage systems will allow better understanding of the relationship between soil biodiversity and changes in crop productivity. There is an overall need to develop biological indicators of soil health, and diagnose soil health status by determining ratios of beneficial and detrimental soil nematodes under different tillage and cropping systems. The development of a rapid and replicable molecular approach using TRFLP will improve the ability of growers to routinely monitor the soil nematode community. Yet TRFLP methods are current commercially unavailable to profile soil nematode communities. The realisation of these goals will allow A&L Laboratories (A&L) to make these tools available to growers so they can assess whether their agronomic practices have made their soil more productive via the development of beneficial soil nematodes. This premise is dependent

了解免耕和传统耕作制度下自由生活的土壤线虫组合的变化，将有助于更好地了解土壤生物多样性和作物生产力变化之间的关系。有一个全面的需要开发土壤健康的生物指标，并诊断土壤健康状况，通过确定在不同的耕作和种植制度下的有益和有害的土壤线虫的比例。一个快速和可复制的分子方法，使用TRFLP的发展将提高种植者的能力，定期监测土壤线虫群落。然而，TRFLP方法是目前商业上不可用的剖面土壤线虫群落。这些目标的实现将使A&L实验室（A&L）能够向种植者提供这些工具，以便他们能够评估他们的农艺措施是否通过开发有益的土壤线虫使土壤更具生产力。这个前提是依赖的