Metabolism and Functions of 4-Aminobutyrate (GABA) in Plants

4-氨基丁酸（GABA）在植物中的代谢和功能

• 批准号: RGPIN-2014-06660
• 负责人: Shelp, Barry
• 金额: $ 2.91万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=567958
• 关键词: Metabolism; Functions; Aminobutyrate; GABA; Plants

Entire sets of gene and protein sequences in plants are rapidly becoming available, and there is increasing need to understand protein structure/function and regulation of metabolic pathways. For the past 2.5 decades, my laboratory has developed internationally recognized leadership in the metabolism and function(s) in plants, of the amino acid 4-aminobutyrate (GABA) and the short-chain fatty acid 4-hydroxybutyrate (GHB), which closely resembles GABA. They are endogenous constituents of the mammalian nervous system, wherein GABA plays a role in neural transmission and development, and functions through interactions with specialized receptors, and GHB serves as a neurotransmitter or neuromodulator postulated to act via a GABA receptor or an independent GHB-specific receptor. The physiological roles of GABA and GHB in plants are not yet clear, but evidence suggests that they are linked to stress, signaling and development. It is becoming clear that GABA metabolism interacts with various biochemical processes and that gene-dependent and -independent processes are involved in its regulation. Elucidation of primary stress- and development-specific responses of the GABA pathway and their interplay with the C/N network and various signaling pathways requires time course experiments and simultaneous monitoring of the relevant genes and metabolites in wild-type plants and genetic mutants. Our current research focuses on the model plant Arabidopsis thaliana because of the availability of molecular reagents, as well as apple (Malus x domestica) fruit because they are prone to physiological injury during controlled atmosphere storage, an abiotic stress. We also study rice (Oryza sativa) and tomato (Solanum lycopersicum) for comparative purposes and for commercial interest. Our approach is innovative and integrative, using physiological, biochemical, and biotechnological methods to identify and understand the regulation of genes/proteins and pathways associated with these metabolites. Our results not only advance fundamental knowledge, but also suggest potential strategies for engineering/developing abiotic and biotic stress resistance and developmental changes in important crop plants.

植物中的整套基因和蛋白质序列正在迅速变得可用，并且越来越需要了解蛋白质结构/功能和代谢途径的调节。在过去的2.5十年中，我的实验室在植物中氨基酸4-氨基丁酸（GABA）和短链脂肪酸4-羟基丁酸（GHB）的代谢和功能方面取得了国际公认的领导地位，这与GABA非常相似。它们是哺乳动物神经系统的内源性成分，其中GABA在神经传递和发育中起作用，并通过与专门受体的相互作用发挥功能，GHB作为神经递质或神经调质，被假定通过GABA受体或独立的GHB特异性受体起作用。GABA和GHB在植物中的生理作用尚不清楚，但有证据表明它们与压力，信号和发育有关。越来越清楚的是，GABA代谢与各种生化过程相互作用，基因依赖性和非依赖性过程参与其调节。阐明的主要压力和发展的具体响应的GABA途径和它们的相互作用与C/N网络和各种信号转导途径需要时程实验和相关基因和代谢产物在野生型植物和遗传突变体的同时监测。我们目前的研究重点是模式植物拟南芥，因为分子试剂的可用性，以及苹果（苹果）水果，因为他们很容易在气调贮藏，非生物胁迫的生理损伤。我们还研究了水稻（水稻）和番茄（番茄）的比较目的和商业利益。我们的方法是创新和综合的，使用生理，生化和生物技术方法来识别和理解与这些代谢物相关的基因/蛋白质和途径的调节。我们的研究结果不仅推进了基础知识，而且还提出了工程/发展非生物和生物胁迫抗性和重要作物发育变化的潜在策略。