Basic studies on biocompatible polymers

生物相容性聚合物的基础研究

• 批准号: 238852-2011
• 负责人: Brooks, Donald
• 金额: $ 2.55万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=570507
• 关键词: Basic; studies; biocompatible; polymers

Basic Studies on Biocompatible Polymers Coating synthetic materials with polymer 'brushes' has been found to resist but not eliminate rejection reactions that occur when unprotected foreign material contacts blood or tissues. We propose basic studies on some polymer systems we are using to (a) construct more biocompatible blood bags and (b) to replace human serum albumin (HSA) or deliver drugs with hyperbranched polyglycidol (HPG) that shares many of HSA's properties. POLYMER BRUSHES: Brushes refer to surfaces from which soluble polymers are grown at sufficiently high density that they are forced into an unusually extended, high energy structure. We have found that they can strongly affect the degree and type of protein adsorption that occurs when any material is exposed to blood or complex mixtures of proteins, rejecting to varying degrees many protein species at particular brush densities. We plan basic studies on brushes grown on both polystyrene latex particles and on flat polyvinyl chloride or silica chips to better understand these interactions. Specifically we will use AFM with 'colloidal' tips to study the properties of brush/protein systems; compare these to contact angle studies using phase separated aqueous two phase polymer mixtures, a parameter that is extremely sensitive to macromolecular structures on surfaces; utilize the new UBC TOF-SIMS instrument to study the composition of mixed protein films on brushes exposed to protein mixtures and understand the role of radius of curvature on brush behavior. HPGs: Hyperbranched polymers act somewhat like proteins because of their compact solution configuration and can be easily derivatized via the many OH groups available. Hydrophobic binding is critical for an HSA mimic and for drug delivery; detailed studies of this kind of reaction will be made as a function of structural properties as well as a thermodynamic study of mixtures containing HPGs to examine their interactions with water, other polymers and proteins. Using various HPG derivatives we will determine the access a second macromolecular species has to the interior of an HPG molecule and how to control it.

生物相容聚合物的基础研究 人们已经发现，在合成材料上涂上聚合物“刷子”可以防止但不能消除当无保护的异物接触血液或组织时发生的排斥反应。我们建议对我们正在使用的一些聚合物系统进行基础研究，以(A)构建更具生物相容性的血袋和(B)取代人血清白蛋白(HSA)或使用具有许多HSA特性的超支化聚缩水甘油(HPG)来输送药物。聚合物刷子：刷子是指以足够高的密度生长可溶聚合物的表面，使它们被迫形成异常延伸的高能结构。我们发现，当任何材料暴露在血液或蛋白质的复杂混合物中时，它们可以强烈影响蛋白质吸附的程度和类型，在特定的刷子密度下，它们在不同程度上排斥许多蛋白质种类。我们计划对聚苯乙烯乳胶颗粒和平板聚氯乙烯或硅片上生长的刷子进行基础研究，以更好地了解这些相互作用。具体地说，我们将使用带有“胶体”尖端的AFM来研究刷子/蛋白质体系的性质；将这些研究与使用相分离的两相聚合物混合物的接触角研究进行比较，该参数对表面的大分子结构极其敏感；利用新的UBC TOF-SIMS仪器来研究接触到蛋白质混合物的刷子上混合蛋白质膜的组成，并了解曲率半径对刷子行为的作用。HPG：超支化聚合物的作用有点像蛋白质，因为它们的溶液结构紧凑，可以很容易地通过许多可用的羟基进行衍生化。疏水结合对于HSA模拟物和药物输送是至关重要的；将详细研究这种反应作为结构性质的函数，以及含有HPG的混合物的热力学研究，以检查它们与水、其他聚合物和蛋白质的相互作用。使用不同的HPG衍生物，我们将确定第二个大分子物种进入HPG分子内部的途径以及如何控制它。