The expression and function of small stress proteins in uterine smooth muscle during pregnancy

妊娠期子宫平滑肌小应激蛋白的表达及功能

• 批准号: 250218-2012
• 负责人: MacPhee, Daniel
• 金额: $ 2.04万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=570849
• 关键词: expression; function; small; stress; proteins

The uterus is a female organ that houses the developing fetus during pregnancy. This organ contains a smooth muscle component named the myometrium and during pregnancy the myometrium goes through a program of differentiation. The rat has been well utilized as an animal model to study this myometrial programming. During rat pregnancy the myometrium is characterized by a Proliferative Phase, a Synthetic Phase, a Contractile Phase, a Labour Phase, and lastly, a post-partum Involution Phase. Each one of these phases of differentiation is characterized by changes in the muscle cells at the molecular and cellular level. Overall, the consequence of this whole process is the production of a tissue that can generate precisely coordinated and powerful contractions (labour) to ensure the timely delivery of a term fetus whose organ systems are sufficiently mature for survival outside the uterus. Small stress or heat shock proteins (Hsp) have important housekeeping roles in cells while heat shock factors (HSF) are master regulators of gene transcription, including that of classical and non-classical heat shock genes. This research program will determine the presence (expression) and regulation of HSF-1, -2, HspB8 and associated proteins in myometrial cells during pregnancy. Moreover, we will determine if any of these molecules have key roles to play in specific phases of myometrial programming. The research will utilize molecular and cell biological tools and procedures, as well as rat experimental models to achieve the research objectives. In total, this research program will create a greater fundamental understanding of the expression, regulation and role of HSF1/2, HspB8 and associated chaperone machinery in myometrial programming during pregnancy.

子宫是女性在怀孕期间容纳发育中的胎儿的器官。这个器官包含一种叫做子宫肌层的平滑肌成分，在怀孕期间，子宫肌层经历了一个分化过程。大鼠已被很好地用作研究这种肌层规划的动物模型。在大鼠怀孕期间，子宫肌层的特征分为增殖期、合成期、收缩期、产程期和最后的产后复旧期。每一个分化阶段都以肌肉细胞在分子和细胞水平上的变化为特征。总的来说，整个过程的结果是产生一个组织，可以产生精确协调和强大的收缩（阵痛），以确保及时分娩足月胎儿，其器官系统足够成熟，可以在子宫外存活。