Universal point-of-care test device for the rapid diagnosis of infectious diseases

传染病已成为公共卫生的主要威胁，每年造成1300多万人死亡，其中 大约50%发生在发展中国家。临床医生依靠实验室来确定病原体负责 这些疾病和启动适当的治疗，这可能需要几天。因此，为了减少这些疾病的健康负担， 需要快速和准确的诊断。我们建议开发一个通用的诊断平台，点的需要（PON） 诊断（在需要时进行），可用于从鼻拭子中快速识别传染病， 血液或尿液，几乎没有动手的时间，可以由非专家进行。我们的设备包括三个主要的 组件;第一，样本递送系统，其分离用于下游应用的病毒或细菌;第二， 样品制备系统，其从病原体释放遗传物质用于检测;以及第三，DNA扩增 和用于鉴定特定病原体相关基因的检测组件。利用这三个要素，我们可以识别出 在不到30分钟的时间内在PON设备中检测感兴趣的病原体，这可以被医生、牙医、兽医 食品加工厂，也可以在家里使用。该诊断平台的成功开发，将对 对全球传染病管理产生重大影响。

Infectious diseases have become a major threat to public health, causing more than 13 million deaths per year, of which approximately 50% occur in developing countries. Clinicians rely on the laboratory to identify the pathogen responsible for these diseases and initiate proper treatment, which can take days. Thus, to reduce the health burden of these diseases, a rapid and accurate diagnosis is required. We propose to develop a universal diagnostic platform for point-of-need (PON) diagnostics (performed where they are needed), which can be used to rapidly identify infectious diseases from nasal swabs, blood or urine with little hands-on time that can be performed by non-experts. Our device consists of three main components; first, a specimen delivery system, which isolates the virus or bacteria for downstream applications; second, a sample preparation system, which releases genetic material from the pathogens for detection; and third, a DNA amplification and detection component to identify specific pathogen-associated genes. Using these three components, we can identify any pathogen-of-interest in a PON device in less than 30 minutes, which can be employed by physicians, dentists, veterinarians, food-processing plants, and also for at-home use. The successful development of this diagnostic platform will have a significant impact on the management of infectious diseases worldwide.