Oligodeoxynucleotides containing CpG motifs (CpG-ODN) as an alternative to antibiotics in the broiler chicken industry

含有 CpG 基序的寡脱氧核苷酸 (CpG-ODN) 作为肉鸡行业抗生素的替代品

• 批准号: 478086-2014
• 负责人: Gomis, Susantha
• 金额: $ 7.94万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=579287
• 关键词: Oligodeoxynucleotides; containing; CpG; motifs; ODN

The use of antibiotics to control early broiler chick mortality was discontinued from May, 2014 in all Canadian broiler chick hatcheries. Hence, alternatives to antibiotics are a priority in the chicken industry. The emergence and spread of antimicrobial resistance are complex problems driven by numerous interconnected factors, thus, diseases in animals has focused attention on immune based control methods. Vaccines are a well-known method to activate the immune system for improved disease protection, but they do not exist for some diseases and may not be effective when an immediate protective effect is required e.g., in the face of a disease outbreak. Therefore, alternate strategies to produce protective immunity are required. The "first responder" or innate component of the immune system detects an infection by recognizing features characteristic of the infectious organisms as "danger" signals. One of these danger signals is a pattern characteristic of bacterial DNA, known as CpG motifs (CpG-ODN). We have demonstrated a significant protective effect of CpG-ODN against lethal bacterial infections in chickens. This proposed research program will study the utility of CpG-ODN in neonatal broiler chickens at the poultry hatchery by delivering CpG-ODN through mucosal surfaces of the respiratory system. We will administer CpG-ODN in neonatal broiler chickens at the hatchery immediately following hatching, thus avoiding the complicating effects of other stimuli on the immune system.

从2014年5月起，所有加拿大肉鸡孵化场停止使用抗生素控制早期肉鸡死亡率。因此，抗生素的替代品是养鸡业的优先事项。抗菌素耐药性的出现和传播是由许多相互关联的因素驱动的复杂问题，因此，动物疾病已经将注意力集中在基于免疫的控制方法上。疫苗是一种众所周知的激活免疫系统以改善疾病保护的方法，但它们对某些疾病并不存在，并且当需要立即保护作用时可能无效，例如，面对疾病的爆发因此，需要替代策略来产生保护性免疫。免疫系统的“第一反应者”或先天组分通过识别感染性疾病的特征来检测感染。 生物体作为“危险”信号。这些危险信号之一是细菌DNA的特征模式，称为CpG基序（CpG-ODN）。我们已经证明了CpG-ODN对鸡的致死性细菌感染具有显著的保护作用。本研究计划将探讨CpG-ODN透过呼吸系统黏膜表面传递给家禽孵化场新生肉鸡的效用。将CpG-ODN应用于新生肉鸡 在孵化后立即在孵化场的鸡，从而避免了其他刺激对免疫系统的复杂影响。