Identification of cranberry pathogens through a multiplex PCR approach

增加蔓越莓疾病的知识是资本的重要性，以确保持续生产 优质水果水果病原体在不同的生产地理区域越来越受到关注。在 在魁北克，虽然蔓越莓疾病的存在是罕见的，在过去，不幸的是，疾病的增加， 困扰着这个行业。种植者现在更普遍地必须做多达三种杀菌剂的应用，以控制 不同的疾病。对于水果加工商来说，在供应链中移除有缺陷的水果既成本高昂， 耗时且影响成品的供应。几个人的精确识别 导致果实腐烂的病原体是复杂的、繁琐的和不准确的。这种情况阻止使用 适当的控制措施。在过去的六个月里，我们开发出了七种特异性引物 蔓越莓最具破坏性的真菌体外实验证实，多重PCR 这种方法可以区分每种真菌的存在。在这个项目中，与Ocean Spray合作， 目的是证明多重PCR方法可以精确诊断致病性 在魁北克的整个生产地区，蔓越莓果实腐烂的病原体。一个精确的知识 攻击蔓越莓果实的病原体将为魁北克（以及加拿大和美国）种植者提供适应性的 控制解决方案，这将有利于整个国家的海洋喷雾和蔓越莓种植者， 提高产量，减少农药使用量和果腐病损失。

Increased knowledge of cranberry diseases is of capital importance to insure the continued production of quality fruits. The fruit pathogens are an increasing concern in the different geographic areas of production. In Quebec, while the presence of cranberry diseases was rare in the past, an unfortunate increase in diseases has plagued the industry. Growers more commonly now have to do up to three fungicide applications to control different diseases. For fruit handlers, the removal of defective fruit in the supply chain is both costly and time-consuming and affects the supply of the finished product. The precise identification of the several pathogens responsible for fruit rot is complex, cumbersome and inaccurate. This situation prevents the use of adequate control measures. Over the last six months, we were able to develop primers that are specific to seven of the most damaging fungi affecting cranberry. In vitro experiments have confirmed that a multiplex PCR approach can discriminate the presence of each fungus. In this project, in collaboration with Ocean Spray, the objective is to demonstrate that the multiplex PCR approach can lead to a precise diagnostic of the causal agent(s) of cranberry fruit rot throughout the area of production in Quebec. A precise knowledge of the pathogens attacking cranberry fruit will provide Quebec (and Canadian and American) growers with adapted control solutions that will benefit both Ocean Spray and cranberry growers throughout the country through yield increment and reduction of pesticide usage and losses incurred by fruit rot.