Designing industrial antifungal formulations of silver nanoparticles: mechanisms of antimicrobial action in C. albicans

关闭解决方案公司（萨斯卡通，SK）已经确定，银纳米粒子具有有效的抗真菌 活性，这取决于颗粒与真菌细胞的相互作用。关闭解决方案公司缺乏 在真菌生物学的专业知识和必要的设备，深入研究这些发现。这种伙伴关系 将大大有利于关闭解决方案公司。通过研究来更好地理解 潜在的银纳米颗粒抗真菌活性。公司联络员将参与指导和 执行将在关闭解决方案实验室进行的研究部分，包括 银纳米颗粒制剂的合成、物理和化学表征。制备物将 最初用于研究和培训Dahms实验室的研究助理，他们将 然后进行合成。关闭解决方案公司的技术人员。还将提供专业知识， 纳米材料领域，并将使用关闭解决方案的微生物实验室进行免费的 根据需要进行抗真菌活性实验和测定。 Dahms集团的独特定位是应用他们的新型高内容，原子力激光扫描 共聚焦显微镜分析，以帮助了解银抗真菌活性的机制。等 测定允许同时追踪细胞内（即，代谢，应激反应）和细胞外 （形态学、超微结构、细胞力学）参数实时作为活真菌响应纳米颗粒 治疗。研究助理将领导真菌研究，解释并提供她的数据关闭 解决方案谁将调整配方进行进一步的测试。这种反复的反馈将帮助我们理解 潜在的抗真菌机制，最终将告知配方更大的功效。

Shutout Solutions Inc. (Saskatoon, SK) has determined that silver nanoparticles possess potent antifungal activity and that is dependent on the interaction of the particles with fungal cells. Shutout Solutions Inc. lacks expertise in fungal biology and requisite equipment for the in-depth study of these discoveries. This partnership will greatly benefit Shutout Solutions Inc. by permitting research to better understand the mechanisms underlying silver nanoparticle antifungal activity. The company liaison will be involved in directing and performing the portion of the research to be conducted at Shutout Solution's laboratories, including the synthesis, physical and chemical characterization of silver nanoparticle preparations. The preparations will initially be used for research and training the research associate from the Dahms laboratory, who will subsequently conduct the synthesis. The technician at Shutout Solutions Inc. will also provide expertise in the field of nanomaterials and will use Shutout Solution's microbiology laboratory to conduct complimentary experiments and assays of antifungal activity as required. The Dahms group is uniquely positioned to apply their novel high-content, atomic force-laser scanning confocal microscopy assays to help understand the mechanisms underlying silver antifungal activity. Such assays allow simultaneous tracking of intracellular (i.e., metabolism, stress responses) and extracellular (morphology, ultrastructure, cell mechanics) parameters in real-time as live fungi respond to nanoparticle treatments. The research associate will lead the fungal studies, interpret and provide her data to Shutout Solutions who will adjust formulations for further testing. This iterative feedback will help us understand the underlying antifungal mechanisms that will ultimately inform formulations for greater efficacy.