Evaluation of the cytotoxic and growth inhibitory effects and synergism of natural plant-derived mixtures in prostate cancer cells.

评估天然植物来源的混合物在前列腺癌细胞中的细胞毒性和生长抑制作用以及协同作用。

• 批准号: 484612-2015
• 负责人: Sanderson, Thomas
• 金额: $ 1.82万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=584428
• 关键词: Evaluation; cytotoxic; growth; inhibitory; effects

The objective of this proposal is to determine the potential anticancer actions of six natural plant-derived extracts by determining their ability to kill prostate cancer cells or inhibit their growth. Prostate cancer is an important burden on society as it is the most frequent cancer diagnosed in men and the second leading cause of cancer-related death of the aging male. Prostate cancer is usually initially androgen-dependent and treatable with antiandrogen therapy. However, often prostate cancer progresses to an androgen-independent state that is drug-resistant and poorly treatable, resulting in metastases and death of the patient. The frequency of prostate cancer is strongly influenced by nutrition, with a diet high in plant-derived foods acting protectively. The long latency period in the development of prostate tumors makes this disease particularly suitable for targeting by nutritional supplements, such as natural plant extracts. We propose to determine the ability of six plant extracts to kill prostate cancer cells with properties reflecting various stages of the disease, including early-stage androgen-dependent and late-stage androgen-independent tumor cells. The plant extracts will be supplied by our collaborating company Idunn Technologies. The cellular mechanisms of cell death will be investigated to determine exactly how the plant extracts kill the cells. In addition, various mixtures of natural plant-derived extracts will be tested to determine potential synergistic effects on prostate cancer cell death. If it is found that different plant extracts kill prostate cancer cells via different cell death pathways, it is possible that a combined exposure to two or more extracts may result in greater than additive effects; this approach may also prevent tumor cells from gaining resistance against on single treatment. The findings of this proposal will provide essential information on the potential for certain plant extracts or mixtures of these extracts to act as effective chemopreventive or chemotherapeutic agents, particularly in the fight against drug-resistant prostate cancer.

本提案的目的是确定六种天然植物来源的潜在抗癌作用， 通过测定其杀死前列腺癌细胞或抑制其生长的能力来确定提取物。前列腺癌是一种 这是社会的一个重要负担，因为它是男性中最常见的癌症，也是导致癌症的第二大原因。 老年男性的癌症相关死亡。前列腺癌最初通常是雄激素依赖性的，是可以治疗的 抗雄激素治疗然而，前列腺癌通常进展到雄激素非依赖性状态， 耐药性和治疗效果差，导致患者转移和死亡。前列腺的频率 癌症受到营养的强烈影响，植物性食物含量高的饮食具有保护作用。长 前列腺肿瘤发展中的潜伏期使得这种疾病特别适合于靶向治疗， 营养补充剂，如天然植物提取物。我们建议确定六种植物提取物的能力， 以杀死具有反映疾病各个阶段的特性的前列腺癌细胞，包括早期阶段， 雄激素依赖性和晚期雄激素非依赖性肿瘤细胞。植物提取物将由 我们的合作公司Idunn Technologies。将研究细胞死亡的细胞机制， 确定植物提取物是如何杀死细胞的此外，还可以使用天然植物衍生的各种混合物， 将测试提取物以确定对前列腺癌细胞死亡的潜在协同作用。如果发现 不同的植物提取物通过不同的细胞死亡途径杀死前列腺癌细胞， 暴露于两种或更多种提取物可能会产生比相加效应更大的影响;这种方法还可以防止 肿瘤细胞对单一治疗产生耐药性。这项建议的结果将提供 关于某些植物提取物或这些提取物的混合物作为有效成分的潜力的基本信息 化学预防剂或化学治疗剂，特别是在对抗耐药性前列腺癌中。