Development of NMR methods to map peptide:Nanoparticle interactions

布鲁克公司是高场核磁共振(核磁共振)光谱仪的领先制造商，并热衷于扩大高分辨率核磁共振方法在多肽-材料相互作用研究中的应用，这些相互作用调节蛋白质在表面的吸附。生物分子吸附被认为是未来高场核磁共振应用的一个新兴领域，因为多肽与材料表面之间的相互作用在从生物材料到生物医学设备和传感器的广泛工业应用中具有相关性。然而，我们目前对蛋白质-物质相互作用的了解充其量也是匮乏的。哪些蛋白质残基与材料表面相互作用？当蛋白质吸附到材料中时，会发生什么样的构象和动力学变化？核磁共振波谱是一种理想的 由于核磁共振硬件的最新发展，包括高场磁铁和更高的灵敏度，解决这些核心问题的技术。然而，为了确定蛋白质吸附的一般分子原理，有必要进一步发展核磁共振方法来绘制原子分辨率下的蛋白质-材料相互作用图。因此，我们建议开发高分辨率的核磁共振方法来绘制多肽与材料的相互作用图。布鲁克加拿大公司将直接受益于这些新的核磁共振方法的开发，因为它们将在工业和学术领域为高场核磁共振光谱仪打开一个新的市场。例如，开发、生产和销售生物制药的生物医药公司将利用开发的核磁共振技术来改进其产品和/或用于质量控制目的。布鲁克加拿大公司还将受益于直接获得样品制备、HQP和高场核磁共振 麦克马斯特的磁铁既可用于开发，也可用于演示，以满足布鲁克潜在客户的需求。我们建议开发的核磁共振实验也有望吸引其他从事材料科学的学术实验室的兴趣。因此，拟议的工作将扩大布鲁克加拿大公司在私营和公共部门的客户基础。

Bruker is a leading manufacturer of high-field Nuclear Magnetic Resonance (NMR) spectrometers and is keenly interested in expanding the application of high-resolution NMR methods to the investigation of peptide-material interactions mediating protein adsorption at surfaces. Biomolecular adsorption is recognized as an emerging field of future high-field NMR applications due to the relevance of the interactions between peptides and material surfaces for a wide range of industrial applications, ranging from biomaterials to biomedical devices and sensors. Yet, our current knowledge of protein - material interactions is at best scant. Which protein residues interact with material surfaces? What conformational and dynamical changes occur in proteins upon adsorption into materials? Nuclear Magnetic Resonance (NMR) spectroscopy is an ideal technique to address these central questions thanks to recent developments in NMR hardware, including high-field magnets and improved sensitivity. However, further development of NMR methods to map protein - material interactions at atomic resolution is necessary in order to define general molecular principles of protein adsorption. We therefore propose to develop high-resolution NMR methods to map the interactions of peptides with materials. Bruker Canada will directly benefits from the development of these new NMR methods, because they will open a new market for high-field NMR spectrometers, both in the industrial and academic sectors. For instance, biomedical companies that develop, produce and market biopharmaceuticals will take advantage of the developed NMR technologies for either improving their products and/or for quality control purposes. Bruker Canada will also benefit from direct access to sample preparation, HQP and high-field NMR magnets available at McMaster for both development and "demo" purposes for potential Bruker customers. The NMR experiments that we propose to develop are also expected to attract the interest of other academic laboratories working on material science. The proposed work will therefore expand the customer base of Bruker Canada, in both the private and public sectors.