Development of high throughput PCR-based pathogen screening diagnostic test for Cannabis production systems

为大麻生产系统开发基于高通量 PCR 的病原体筛查诊断测试

• 批准号: 485350-2015
• 负责人: Charles, Trevor
• 金额: $ 1.82万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=584891
• 关键词: Development; throughput; PCR; based; pathogen

The yield and quality of medical cannabis is of critical importance. A major concern is the detection of potential contaminating human and plant pathogens at early stages, before crop loss or damage ensues. While the procedure for testing of chemical contaminants is well established, microbiological contaminants are more difficult, unpredictable, and complex. DNA-based methods have become the preferred approach to detection of microbiological contaminants. This project is aimed at developing rapid and accurate protocols for pathogen screening in closed-environment cannabis production systems. These protocols will be based on polymerase chain reaction (PCR), an established molecular genetic tool, using custom DNA PCR primer probes. It is envisioned that the outcome of this project will yield a suitable pathogen screening procedure that can be implemented for in-house monitoring at the MedReleaf production facility.

医用大麻的产量和质量至关重要。一个主要的问题是检测潜在的 在作物损失或损害加剧之前的早期阶段污染人类和植物病原体。而 化学污染物的测试程序已经建立，微生物污染物更多 困难、不可预测、复杂。基于DNA的方法已成为检测 微生物污染物。该项目旨在开发快速准确的病原体检测方案， 在封闭环境的大麻生产系统中进行筛选。这些方案将基于聚合酶 链反应（PCR），一种已建立的分子遗传学工具，使用定制的DNA PCR引物探针。是 设想这个项目的结果将产生一个合适的病原体筛选程序， 在MedReleaf生产机构进行内部监测。