Characterization of two strains of bacteria for phosphate solubilisation from hog manure solids and for plant growth promotion

养猪场的粪便对农作物来说是有益的土壤改良剂，因为它的氮和磷含量都很高。大多数磷酸盐是不溶性的，由于施用制度是基于氮含量的，随着时间的推移，这种不溶性磷酸盐的大部分会在土壤中积累。这限制了每年可施用于土壤的猪粪量。在马尼托巴省，养猪场附近的一些田地已经达到了允许添加磷酸盐的极限，远距离运输粪肥成本过高，限制了其在更广泛地区的应用。使用固体酸发酵，然后在磷酸盐增溶菌（PSB）的存在下进行好氧消化，应导致磷酸盐从粪便固体中增溶和分离。KGS希望研究从这种处理中产生两种土壤肥力增强产品的可行性：1)高可溶性磷酸盐茶，可以直接作为土壤肥料施用，也可以作为鸟粪石重新沉淀，以便有效运输；2)富含PSB的发酵固体可以作为土壤调节剂和有益细菌的来源，用于原位磷酸盐增溶和动员到田间作物的根部。除了磷酸盐增溶外，一些PSB还具有促进植物生长的特性。为KGS集团工程公司分离的两株细菌可能具有增溶磷酸钙的作用，并可能具有促进植物生长的功效。目前的Engage提案的目的有三个方面：1)表征不同不溶性形式的磷酸盐矿物（磷酸钙，鸟粪石）在不同碳源和不同介质组成下的生长和增溶作用。2)对两株菌株的基因组进行注释和比较，为观察到的菌株的生长特性提供合理的认识，并为这些菌株的遗传潜力提供进一步的见解。3)比较马尼托巴省相关作物（油菜籽、大豆、玉米等）对植物相关磷酸盐增溶的促进能力和促进生长的能力，确定这些品系在根际的竞争能力。

Manure from hog farming can be a beneficial soil amendment for agricultural crops, since it is high in both nitrogen and phosphate. Most of the phosphate is insoluble, and since the application regime is based on nitrogen content, much of this insoluble phosphate accumulates in soil over time. This limits the yearly amount of hog manure that can be applied to the soil. In Manitoba, some of the fields near hog farms have reached the limit of allowable added phosphate, and transport of manure any great distance becomes cost prohibitive limiting its application to a wider region. Use of an acid fermentation of the solids, followed by an aerobic digestion in the presence of phosphate solubilizing bacteria (PSB) should lead to the solubilization and separation of the phosphate from the manure solids. KGS wants to investigate the feasibility of generating two soil fertility enhancing products from this treatment: 1) a high soluble phosphate tea which can be applied directly as a soil fertilizer or re-precipitated as struvite for effective transportation, and 2) the fermented solids, enriched with PSB can act as a soil conditioner and a source of beneficial bacteria for in situ phosphate solubilization and mobilization to the roots of field crops. Several PSB also have plant promotion properties, beyond phosphate solubilization. Two strains of bacteria isolated for KGS Group Engineering may be effective at solubilizing calcium phosphate and may have some efficacy in promoting plant growth. The purpose of the current Engage proposal is 3 fold: 1) To characterize the growth and solubilization from different insoluble forms of phosphate minerals (calcium phosphate, struvite) using different carbon sources and different medium composition. 2) To annotate and compare the genomes of the 2 strains in order to provide a rational understanding for the growth characteristics observed and provide further insights into the genetic potential of these strains that could be exploited. 3) To compare the plant promotion capabilities for plant associated phosphate solubilization as well as growth promotion capabilities using Manitoba-relevant crops (canola, soybean, corn, etc.), and determine the competitiveness of these strain in the rhizosphere.