Quantum Chemical Investigations of the Inner Mitochondrial Membrane Biochemistry
线粒体内膜生物化学的量子化学研究
基本信息
- 批准号:RGPIN-2015-03731
- 负责人:
- 金额:$ 2.04万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2015
- 资助国家:加拿大
- 起止时间:2015-01-01 至 2016-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The mitochondrion is a complex and compartmentalized eukaryotic cell organelle responsible for cellular respiration in a multicellular organism that is, burning foodstuffs to produce the energy necessary for sustaining life. It consists of an inner matrix (the location of the Kerbs cycle) enclosed by two membranes: An outer smooth and permeable membrane, and an inner corrugated and highly selective one. The inner membrane hosts an array of connected chemical reactions that use the energy released in exergonic electron transfers to pump protons out of the matrix and into the gap between the membranes. This proton imbalance is equivalent to stored energy due to the combined concentration and electrical gradients. When protons re-enter the matrix this energy is released. If the protons re-enter through a "leak" in the membrane (e.g. due to uncouplers), the released energy is dissipated as heat and the cell gains nothing. To sustain life (by producing ATP), the protons must re-enter through a specific channel within ATP-synthase which captures the energy to drive the endergonic reaction ADP+Pi--> ATP. The proposed research has two broad goals: (a) Discovering some aspects of the rich chemistry that happens inside the inner mitochondrial membrane using the modern tools of quantum chemistry, and (b) use the mitochondrial systems to develop, test, and improve emerging methodologies in applied quantum chemistry. With regards to (a), several aspects of inner mitochondrial membrane biochemistry will be explored computationally, for example, the effect of the strong electric field across the membrane on the reactivity of membrane-bound species and its relation to the production of the superoxide radicals implicated in accelerating cell damage, degenerative diseases, and programmed cell death. With respect to (b) there are two sub-branches. The first is to improve and broaden the applicability of a recently proposed molecular fingerprinting tool by predicting the properties of uncouplers with known activities in quantitative structure-activity relationship (QSAR)-type studies. Second, the reconstruction of approximate ab initio quality electron density and electrostatic potential of a gigantic protein, namely, ATP-synthase, the (partial) low resolution (9 A) structure of which has just been published. This structure will first be completed through homology modeling and the careful consideration of chemical constraints. To start, the missing amino acids and missing atoms will be added followed by force field geometry optimizations. The refined geometry will then be used to define the necessary kernel fragments that will be combined to reconstruct the electron density and electrostatic potential of the entire protein at an ab initio level, approximating in months elusive exact results that, in principle, should take more than the age of the Universe to calculate with present-day computers.
细胞器是一种复杂的真核细胞细胞器,负责多细胞生物体中的细胞呼吸,即燃烧食物以产生维持生命所需的能量。它由两层膜包围的内部基质(Kerbs循环的位置)组成:一层是光滑的可渗透膜,另一层是波纹状的高选择性膜。内膜上有一系列相互关联的化学反应,这些反应利用放能电子转移中释放的能量将质子泵出基质,进入膜之间的差距。这种质子不平衡相当于由于组合的浓度和电梯度而储存的能量。当质子重新进入矩阵时,这种能量被释放。如果质子通过膜中的“泄漏”(例如,由于解偶联剂)重新进入,则释放的能量以热量的形式耗散,并且电池什么也得不到。为了维持生命(通过产生ATP),质子必须通过ATP合酶内的特定通道重新进入,ATP合酶捕获能量以驱动吸能反应ADP+Pi-> ATP。拟议的研究有两个广泛的目标:(a)使用量子化学的现代工具发现线粒体内膜内发生的丰富化学的某些方面,以及(B)使用线粒体系统开发,测试和改进应用量子化学的新兴方法。关于(a),将通过计算探索线粒体内膜生物化学的几个方面,例如,跨膜强电场对膜结合物质的反应性的影响及其与加速细胞损伤、退行性疾病和程序性细胞死亡中涉及的超氧化物自由基的产生的关系。关于(B),存在两个子分支。第一个是改进和扩大最近提出的分子指纹工具的适用性,通过预测解偶联剂与已知的活动在定量构效关系(QSAR)类型的研究。第二,重建近似从头计算质量的电子密度和静电势的一个巨大的蛋白质,即ATP合酶,(部分)低分辨率(9 A)的结构,其中刚刚公布。这个结构将首先通过同源性建模和仔细考虑化学约束来完成。首先,将添加缺失的氨基酸和缺失的原子,然后进行力场几何优化。精确的几何结构将被用来定义必要的核心片段,这些片段将被结合起来,在从头开始的水平上重建整个蛋白质的电子密度和静电势,在几个月内近似难以捉摸的精确结果,原则上,应该需要比宇宙年龄更长的时间来计算。
项目成果
期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
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Matta, Cherif其他文献
Matta, Cherif的其他文献
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{{ truncateString('Matta, Cherif', 18)}}的其他基金
Theoretical Investigations of some Outstanding Problems in Biophysical Chemistry
生物物理化学若干突出问题的理论研究
- 批准号:
RGPIN-2022-04842 - 财政年份:2022
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Quantum Chemical Investigations of the Inner Mitochondrial Membrane Biochemistry
线粒体内膜生物化学的量子化学研究
- 批准号:
RGPIN-2015-03731 - 财政年份:2021
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Quantum Chemical Investigations of the Inner Mitochondrial Membrane Biochemistry
线粒体内膜生物化学的量子化学研究
- 批准号:
RGPIN-2015-03731 - 财政年份:2020
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Quantum Chemical Investigations of the Inner Mitochondrial Membrane Biochemistry
线粒体内膜生物化学的量子化学研究
- 批准号:
RGPIN-2015-03731 - 财政年份:2019
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Quantum Chemical Investigations of the Inner Mitochondrial Membrane Biochemistry
线粒体内膜生物化学的量子化学研究
- 批准号:
RGPIN-2015-03731 - 财政年份:2018
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Quantum Chemical Investigations of the Inner Mitochondrial Membrane Biochemistry
线粒体内膜生物化学的量子化学研究
- 批准号:
RGPIN-2015-03731 - 财政年份:2017
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Quantum Chemical Investigations of the Inner Mitochondrial Membrane Biochemistry
线粒体内膜生物化学的量子化学研究
- 批准号:
RGPIN-2015-03731 - 财政年份:2016
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
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