Dissection of Subcellular Responses and Interactions in Plants

植物亚细胞反应和相互作用的剖析

• 批准号: RGPIN-2015-03917
• 负责人: Mathur, Jaideep
• 金额: $ 2.77万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=589468
• 关键词: Dissection; Subcellular; Responses; Interactions; Plants

All living organisms respond to environmental cues and employ a variety of sub-cellular strategies for maximizing their chances of survival. Plants, despite their sedentary nature have been especially successful in combating the myriad stresses surrounding them. Plant cells, therefore, have been the research material of choice for my lab where we strive to understand their subtle stratagems for maintaining cellular homeostasis. Research into my long-term goal of understanding the responsiveness of the plant cell was initiated through my first Discovery grant in 2005 and has extended seamlessly into the second grant period with considerable benefit from a Discovery accelerated supplement. During the past grant periods my live-imaging based approach has resulted in a wide variety of basic tools using conventional and photo-convertible fluorescent proteins. The lab has also developed several pioneering techniques for precise targeting and tracking of sub-cellular components and compartments. Most important, keeping our long-term goal in mind, is our discovery and initial characterization of novel sub-cellular phenomenon involving rapid membrane extensions from different organelles (Sinclair et al., 2009; Mathur et al., 2013; Schattat et al. 2012). The transient phenomena reported by us appear to be common to all eukaryotes and are very often involved in bringing a stressed cell back to equilibrium quickly as well as allowing it to be better prepared for dealing with recurrent stress. This proposal builds up on my foundational work of the last 10 years and is aimed at uncovering more details about the basic subcellular phenomena identified so far. The four key and complementary areas of exploration defined by me for investigation under the present proposal are: (1). Understanding the mechanisms involved in the formation of organelle extensions. (2). Understanding the role of organelle extensions in inter-organelle trafficking. (3). Understanding the role of the endoplasmic reticulum (ER) as a physical regulator of organelle shape and interactivity.(4). Understanding the fundamentals of endomembrane-cytoskeletal interactions.

所有活着的有机体都会对环境信号做出反应，并采用各种亚细胞策略来最大化它们的生存机会。植物，尽管它们具有静坐的性质，但在对抗它们周围的无数压力方面尤其成功。因此，植物细胞一直是我实验室的研究材料，在那里我们努力了解它们维持细胞动态平衡的微妙策略。我对了解植物细胞响应性的长期目标的研究是在2005年通过我的第一次发现拨款开始的，并已无缝地延长到第二次拨款期间，并从发现加速补充中获得了相当大的好处。在过去的赠款期间，我的基于实时成像的方法已经产生了使用传统的和光可转换的荧光蛋白的各种基本工具。该实验室还开发了几项开创性的技术，用于精确定位和跟踪亚细胞组件和隔间。最重要的是，牢记我们的长期目标，是我们发现并初步描述了涉及不同细胞器快速膜延伸的新的亚细胞现象(Sclair等人，2009；Mathur等人，2013；Schattat等人)。2012年)。我们所报道的瞬时现象似乎在所有真核生物中都是常见的，并且经常涉及到使应激细胞迅速恢复平衡以及使其为应对反复应激做好更好的准备。这项建议建立在我过去10年的基础性工作基础上，旨在揭示迄今为止已确定的基本亚细胞现象的更多细节。根据本提议，我为调查界定的四个关键和互补的勘探领域是：(1)。了解细胞器延伸形成的机制。(2)。了解细胞器延伸在细胞器间贩运中的作用。(3)。了解内质网(ER)作为细胞器形状和相互作用的物理调节器的作用。了解内膜-细胞骨架相互作用的基本原理。