Equilibrative nucleoside transporters: Post-translational regulation and protein interactions

平衡核苷转运蛋白：翻译后调节和蛋白质相互作用

• 批准号: RGPIN-2015-05470
• 负责人: Hammond, James
• 金额: $ 2.19万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=590451
• 关键词: Equilibrative; nucleoside; transporters; Post; translational

Our body is made up of individual units called 'cells'.  Each cell has a specialized function.  Cells have an outer membrane that forms a barrier to many substances.  In order for cells to take up nutrients from the blood that they need to survive and grow, they must rely on specific molecules (proteins) in the cell membrane to facilitate the passage of these nutrients into the cell.  We are studying one particular type of these "transport" proteins involved in the cellular uptake and release of nucleosides. Nucleosides are a class of substances that are used as the building blocks for DNA and RNA, and are also important for controlling many other aspects of cell function. Cells require different levels of nucleosides at different times, and must adapt to their environment to ensure that the nucleoside concentrations, both inside and outside the cell, are maintained at specific levels. We are studying this cellular adaptation process. Our studies are focused on how cells regulate the number and activity of "nucleoside transporters". An increase or decrease in nucleoside transporter activity has been known to modify blood flow to tissues, protect the heart and brain tissue from damage during transient ischemic episodes, and modify the duration of seizures.  Nucleoside transporters are also important for the activity of a number of anti-cancer and anti-viral drugs that get into the tumour cells or virus-infected cells via these transporters.  A better understanding of how the body regulates nucleoside transporters will enhance our ability to develop better therapies for conditions such as heart failure, seizures, and stroke.  These studies will also enhance our understanding of how the cell regulates its metabolism in response to environmental influences.

我们的身体是由称为“细胞”的个体单位组成的。*每个细胞都有专门的功能。这些细胞有一层外膜，可以对许多物质形成屏障。为了让细胞从血液中摄取它们生存和生长所需的营养，它们必须依靠细胞膜中的特定分子(蛋白质)来促进这些营养物质进入细胞。我们正在研究一种参与细胞摄取和释放核苷的特殊类型的“运输”蛋白。核苷是一类用作构建DNA和RNA的物质，对控制细胞功能的许多其他方面也很重要。细胞在不同的时间需要不同水平的核苷，必须适应其环境，以确保细胞内外的核苷浓度保持在特定的水平。我们正在研究这种细胞适应过程。我们的研究集中在细胞如何调节“核苷转运体”的数量和活性。核苷转运体活性的增加或减少已被认为可以改变流向组织的血液，保护心脏和脑组织免受短暂性脑缺血发作的损害，并改变癫痫发作的持续时间。核苷转运体对一些抗癌和抗病毒药物的活性也很重要，这些药物通过这些转运体进入肿瘤细胞或病毒感染的细胞。更好地了解人体如何调节核苷转运体将增强我们为心力衰竭、癫痫和中风等疾病开发更好疗法的能力。这些研究还将加强我们对细胞如何调节其新陈代谢以应对环境影响的理解。