Upgrade for fluorescence activated cell sorter

荧光激活细胞分选仪升级

• 批准号: RTI-2016-00041
• 负责人: Johnson, Pauline
• 金额: $ 10.84万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591320
• 关键词: Upgrade; fluorescence; activated; cell; sorter

In this grant application, we are requesting an upgrade to a sophisticated instrument known as a ‘fluorescence-activated cell sorter’. We are requesting two additional lasers that will increase the capability of this machine from detecting 6 fluorescent markers to detecting 13. This will greatly enhance its ability to identify rare cells from complex mixtures of cells. For example, it will now be possible to identify rare immune cell populations from the blood and tissues, and will allow the detection of uniquely fluorescent microbes or fluorescently labeled cells from complex mixtures of over 200 million cells. This upgrade is a much-needed feature that will benefit researchers in immunology, stem cell biology, microbiology, chemical enzymology and biological engineering. In addition, these 2 lasers provide the unique ability to measure violet-activated fluorescent probes and red fluorescent proteins that are increasingly used by researchers to label cells. This upgrade will provide a cutting edge cell sorter capable of high throughput sorting of multi-fluorescent samples and will be housed in the University’s Flow Cytometry Facility, accessible to all researchers. The facility provides training, support and expertise that enables researchers to sort samples and develop new innovative applications. For example, the upgraded instrument will be used in genetic screens to identify and isolate rare mutations in bacteria. It will enable immunologists to better understand the mechanisms immune cells employ in response to pathogens, and enable virologists better understand host-pathogen interactions. It will help lead to the optimization of large batch growth conditions required for the development of mammalian stem cells as new biologics, assist in the directed evolution of improved enzymes that provide new catalysts for industrial processes and contribute to the development of new therapeutics. The upgraded instrument will help increase our knowledge of microbial communities in unexplored ecosystems, provide new insights into genetic exchange in bacteria and nutrient induced gene regulation in yeast. It will help provide new insights into genetic imprinting in embryos. This upgraded instrument will thus enhance several research programs and will enable new scientific innovations and advances, all of which will ultimately benefit Canadians. It will help increase knowledge in many areas of biology, fuel innovative discoveries in biotechnology and create new industrial and medical applications. It will also enhance the training and technological capabilities of the many highly qualified personnel associated with our research programs.

在这项拨款申请中，我们要求升级到一种被称为“荧光激活细胞分选仪”的复杂仪器。我们需要两个额外的激光器，以提高这台机器的能力，从6个荧光标记检测到13个。这将极大地增强其从复杂的细胞混合物中识别稀有细胞的能力。例如，现在可以从血液和组织中识别罕见的免疫细胞群体，并将能够从超过2亿个细胞的复杂混合物中检测出独特的荧光微生物或荧光标记的细胞。这一升级是一个亟需的功能，将使免疫学、干细胞生物学、微生物学、化学酶学和生物工程领域的研究人员受益。此外，这两种激光提供了测量紫色激活的荧光探针和红色荧光蛋白的独特能力，研究人员越来越多地使用它们来标记细胞。这次升级将提供一台先进的细胞分选机，能够对多种荧光样本进行高通量分选，并将安装在大学的流式细胞术设施中，所有研究人员都可以使用。该设施提供培训、支持和专业知识，使研究人员能够对样品进行分类并开发新的创新应用程序。例如，升级后的仪器将用于基因筛查，以识别和分离细菌中的罕见突变。它将使免疫学家更好地了解免疫细胞对病原体的反应机制，并使病毒学家更好地了解宿主与病原体的相互作用。它将有助于优化哺乳动物干细胞作为新生物制剂开发所需的大批量生长条件，帮助改进的酶的定向进化，为工业过程提供新的催化剂，并有助于新疗法的开发。升级后的仪器将有助于增加我们对未探索生态系统中微生物群落的知识，为细菌中的基因交换和酵母中营养诱导的基因调控提供新的见解。这将有助于对胚胎中的基因印记提供新的见解。因此，这一升级后的仪器将加强几个研究项目，并使新的科学创新和进步成为可能，所有这些最终都将使加拿大人受益。它将有助于增加生物学许多领域的知识，推动生物技术的创新发现，并创造新的工业和医疗应用。它还将增强与我们的研究计划相关的许多高素质人员的培训和技术能力。