Fast wavelength switching controls and a high sensitivity camera for enhanced imaging

快速波长切换控制和高灵敏度相机可增强成像

• 批准号: RTI-2016-00485
• 负责人: Fairn, Gregory
• 金额: $ 6.49万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591461
• 关键词: Fast; wavelength; switching; controls; sensitivity

Whether a cell is free-living in the environment or part of a layer of cells surrounding an organ or tissue, it must interact and sense its surroundings. Each cell is contained within a bilayer of fats (or lipids) and proteins referred to as the plasma membrane. Interactions between a cell and its surroundings or neighboring cells occur through the proteins and lipids located on the cell's outer surface. The plasma membrane and its many different surface proteins allow each cell to take up or "eat" nutrients such as sugars, dietary fat, and vitamins. The plasma membrane also allows a cell to communicate with other cells by receiving signals in the form of hormones and forming protective barriers such as skin or kidneys. Many of these features are examined and studied by the applicants under the umbrella of cell biology. As cell biologists, it is of paramount importance for us to visualize not only the processes under examination but also the molecules that control these events. In our research, that means visualizing the membrane proteins and lipids that mediate cellular organization, movement, and housekeeping.

无论细胞是在环境中自由生活，还是作为器官或组织周围细胞层的一部分，它都必须与周围环境相互作用并感知周围环境。每个细胞都包含在脂肪（或脂质）和蛋白质的双层中，称为质膜。细胞与其周围或邻近细胞之间的相互作用通过位于细胞外表面的蛋白质和脂质发生。质膜及其许多不同的表面蛋白质允许每个细胞摄取或“吃”营养素，如糖，膳食脂肪和维生素。质膜还允许细胞通过接收激素形式的信号并形成保护屏障（如皮肤或肾脏）与其他细胞进行通信。这些特征中的许多特征由申请人在细胞生物学的保护伞下进行检查和研究。作为细胞生物学家，对我们来说至关重要的是，不仅要可视化所检查的过程，还要可视化控制这些事件的分子。在我们的研究中，这意味着可视化介导细胞组织，运动和管家的膜蛋白和脂质。