Regulation of Lipid Metabolism and Vesicular Trafficking by the Oxysterol Binding Protein Kes1

每个细胞都被一层膜包围，这层膜将细胞与周围的细胞和外部环境隔离开来。每个细胞内部都有更多的膜，这些膜进一步将细胞划分为执行特定功能的单独隔间。复制基因，制造能量，或者清除不需要的或用完的细胞垃圾)。把细胞膜想象成一个球的外面，每个隔室都是大球里面的小球。当一个细胞生长并分裂成另一个细胞时，细胞膜需要被合成，并从细胞内的合成地点运送到细胞表面，以允许细胞生长。细胞膜合成是如何与它们的运输协调的，人们知之甚少。本资助申请中提出的研究旨在确定哪些过程允许细胞协调细胞生长与细胞膜运输到其表面，从而使细胞生长、膜合成和运输适当同步。这是我们目前缺乏的基本细胞生物学课本知识。这项拨款旨在填补我们对细胞生物学理解的主要空白。

Each cell is surrounded by a membrane that isolates the cell from surrounding cells and its external environment. Inside each cell are more membranes that further divide the cell into individual compartments that perform specific functions (eg. duplicating genes, making energy, or getting rid of unwanted or used up cellular garbage). Think of the cell membrane as the outside of a ball and each compartment as smaller balls inside the larger one. As a cell grows and divides to make another cell the membranes of the cell need to be synthesized and transported from their site of synthesis inside the cell to the cell surface to allow for cell growth. How cell membrane synthesis is coordinated with their transport is poorly understood. The research proposed in this grant application aims to determine what the processes that allow a cell to coordinate cell growth with the transport of cell membranes to its surface so that cell growth and membrane synthesis and transport are properly synchronized. This is basic cell biology textbook knowledge that we currently lack. This grant proposes to fill major gaps in our understanding of cell biology.