Systematic functional characterization of new bacterial cell envelope assembly factors

新细菌细胞包膜组装因子的系统功能表征

• 批准号: 436383-2013
• 负责人: ParadisBleau, Catherine
• 金额: $ 2.33万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=596909
• 关键词: Systematic; functional; characterization; new; bacterial

Bacteria are unicellular organisms surrounded by a complex cell envelope. This envelope is essential for bacterial growth and survival as it delimits the cell and protects it against environmental assaults and changes in osmotic pressure. Many of our most successful antibiotics, including penicillin and vancomycin, target cell envelope assembly. Ironically, most of our knowledge about bacterial cell envelopes has come from using these antibiotics as functional probes. This has no doubt biased and limited our view of these complex and diverse structures, suggesting a need for alternative approaches. Actually very little is known about how envelope assembly is regulated and how the synthesis of the different layers is coordinated.

细菌是由复杂的细胞被膜包围的单细胞生物。这种包膜对于细菌的生长和存活是必不可少的，因为它界定了细胞并保护其免受环境攻击和渗透压变化的影响。我们许多最成功的抗生素，包括青霉素和万古霉素，靶细胞包膜组装。具有讽刺意味的是，我们对细菌细胞包膜的大部分了解都来自于使用这些抗生素作为功能探针。这无疑使我们对这些复杂和多样的结构的看法产生偏见和局限，表明需要采取其他办法。实际上，关于包膜组装是如何调节的以及不同层的合成是如何协调的，我们知之甚少。