Elucidating molecular mechanisms by which seeds respond to environmental factors

阐明种子响应环境因素的分子机制

• 批准号: RGPIN-2014-03621
• 负责人: Nambara, Eiji
• 金额: $ 2.48万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=610549
• 关键词: Elucidating; molecular; mechanisms; seeds; respond

Plant hormones are low abundant signal molecules, which significantly influence a variety of processes in plant growth and development. Functional genomics research in cereals has revealed that key genetic loci for yield production and stress responses selected by the classical breeding include a series of hormone metabolism and signaling genes. The plant hormone metabolism is regulated by a number of environmental factors and plays an important role in tuning plant growth in response to changing environment. Current challenge in plant biology and biotechnology is to understand how plants sense the environmental factors and alter the plant hormone metabolism. The Nambara research group studies how plants control cellular concentrations of the plant hormone levels with a focus on abscisic acid (ABA) metabolism. We use Arabidopsis seed germination as a model system, because seeds have sophisticated mechanisms to sense environmental conditions. Mature, dry seeds contain all the components required to resume the plant’s life cycle in an appropriate environment. The seeds sense water, nutrients, temperature, after-ripening, and light to determine if the appropriate germination environment exists, and a change in these external cues alters plant hormone metabolism and signaling, which determine whether seeds germinate or remain dormant. The proposed research aims to elucidate the molecular mechanisms by which seeds sense environmental signals and regulate plant hormone metabolism, with a focus on how nitrate regulates ABA catabolism and seed germination. Nitrate is a major nitrogen source existing in soil and influences plant growth and development as a nutrient and as a signal. As a nutrient, nitrate is assimilated to ammonium and then incorporated into amino acids. As a signal, low nitrate concentration causes rapid reprogramming of genome-wide gene expression, even in mutants defective in nitrate assimilation. Nitrate promotes seed germination in many plant species. The effect of nitrate on germination can be observed in mutants defective in nitrate assimilation. This indicates that it acts as a signal to stimulate germination. My research group previously reported that nitrate activates ABA catabolism and reduces ABA, an inhibitor of germination. Despite recent identification of essential genes for nitrate sensing and signal transduction, the molecular mechanisms by which nitrate regulates ABA catabolism remain largely unknown. The overall goal of this grant is to investigate the molecular and cellular mechanisms involved in nitrate regulation of ABA metabolism and seed germination. My research group has recently identified the novel factor regulating these processes during Arabidopsis seed germination. We will elucidate the molecular function of the regulators in nitrate signaling in seeds. Stable crop yield under nitrate limited condition is a key target for plant biotechnology, because sustainable agricultural practices must limit expensive and potentially polluting nitrate inputs. Our basic research will help to gain a novel solution to increase crop yield and decrease crop losses.

植物激素是一种低丰度的信号分子，对植物生长发育过程中的多种过程具有重要影响。禾谷类作物功能基因组学研究表明，经典育种选择的与产量生产和逆境反应有关的关键基因位点包括一系列激素代谢和信号传导基因。植物激素代谢受许多环境因子的调节，在调节植物生长以响应变化的环境中起着重要作用。当前植物生物学和生物技术面临的挑战是了解植物如何感知环境因素并改变植物激素代谢。南原研究小组研究植物如何控制植物激素水平的细胞浓度，重点是脱落酸（阿坝）代谢。我们使用拟南芥种子萌发作为模型系统，因为种子具有复杂的机制来感知环境条件。成熟的干种子含有在适当环境中恢复植物生命周期所需的所有成分。种子感知水分、营养、温度、后熟和光照，以确定是否存在适当的发芽环境，这些外部信号的变化会改变植物激素代谢和信号传导，从而决定种子是发芽还是保持休眠。该研究旨在阐明种子感知环境信号和调节植物激素代谢的分子机制，重点是硝酸盐如何调节阿坝催化剂和种子萌发。 硝态氮是土壤中的主要氮源，作为营养物质和信号物质影响植物的生长发育。作为一种营养素，硝酸盐被同化为铵，然后被掺入氨基酸中。作为一个信号，低硝酸盐浓度导致全基因组基因表达的快速重编程，即使在硝酸盐同化缺陷的突变体中也是如此。硝酸盐在许多植物中促进种子萌发。在硝酸盐同化缺陷的突变体中可以观察到硝酸盐对发芽的影响。这表明它作为刺激发芽的信号。我的研究小组以前报告说，硝酸盐激活阿坝催化剂，减少阿坝，发芽抑制剂。尽管最近确定的硝酸盐传感和信号转导的必需基因，硝酸盐调节阿坝catalysts的分子机制仍然在很大程度上未知。 这项资助的总体目标是研究硝酸盐调节阿坝代谢和种子萌发的分子和细胞机制。我的研究小组最近已经确定了在拟南芥种子萌发过程中调节这些过程的新因子。我们将阐明种子中硝酸盐信号调节剂的分子功能。硝酸盐限制条件下的稳定作物产量是植物生物技术的关键目标，因为可持续农业实践必须限制昂贵且可能污染的硝酸盐投入。我们的基础研究将有助于获得一种新的解决方案，以提高作物产量，减少作物损失。