Elucidating molecular mechanisms by which seeds respond to environmental factors

阐明种子响应环境因素的分子机制

• 批准号: RGPIN-2014-03621
• 负责人: Nambara, Eiji
• 金额: $ 2.48万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=610549
• 关键词: Elucidating; molecular; mechanisms; seeds; respond

Plant hormones are low abundant signal molecules, which significantly influence a variety of processes in plant growth and development. Functional genomics research in cereals has revealed that key genetic loci for yield production and stress responses selected by the classical breeding include a series of hormone metabolism and signaling genes. The plant hormone metabolism is regulated by a number of environmental factors and plays an important role in tuning plant growth in response to changing environment. Current challenge in plant biology and biotechnology is to understand how plants sense the environmental factors and alter the plant hormone metabolism. The Nambara research group studies how plants control cellular concentrations of the plant hormone levels with a focus on abscisic acid (ABA) metabolism. We use Arabidopsis seed germination as a model system, because seeds have sophisticated mechanisms to sense environmental conditions. Mature, dry seeds contain all the components required to resume the plant’s life cycle in an appropriate environment. The seeds sense water, nutrients, temperature, after-ripening, and light to determine if the appropriate germination environment exists, and a change in these external cues alters plant hormone metabolism and signaling, which determine whether seeds germinate or remain dormant. The proposed research aims to elucidate the molecular mechanisms by which seeds sense environmental signals and regulate plant hormone metabolism, with a focus on how nitrate regulates ABA catabolism and seed germination. Nitrate is a major nitrogen source existing in soil and influences plant growth and development as a nutrient and as a signal. As a nutrient, nitrate is assimilated to ammonium and then incorporated into amino acids. As a signal, low nitrate concentration causes rapid reprogramming of genome-wide gene expression, even in mutants defective in nitrate assimilation. Nitrate promotes seed germination in many plant species. The effect of nitrate on germination can be observed in mutants defective in nitrate assimilation. This indicates that it acts as a signal to stimulate germination. My research group previously reported that nitrate activates ABA catabolism and reduces ABA, an inhibitor of germination. Despite recent identification of essential genes for nitrate sensing and signal transduction, the molecular mechanisms by which nitrate regulates ABA catabolism remain largely unknown. The overall goal of this grant is to investigate the molecular and cellular mechanisms involved in nitrate regulation of ABA metabolism and seed germination. My research group has recently identified the novel factor regulating these processes during Arabidopsis seed germination. We will elucidate the molecular function of the regulators in nitrate signaling in seeds. Stable crop yield under nitrate limited condition is a key target for plant biotechnology, because sustainable agricultural practices must limit expensive and potentially polluting nitrate inputs. Our basic research will help to gain a novel solution to increase crop yield and decrease crop losses.

植物激素是一种低丰度的信号分子，对植物生长发育的多种过程具有重要影响。谷物功能基因组学研究表明，经典育种选择的影响产量和胁迫反应的关键遗传位点包括一系列激素代谢和信号基因。植物激素代谢受多种环境因子的调控，在调节植物生长以适应环境变化中起着重要作用。植物生物学和生物技术目前面临的挑战是了解植物如何感知环境因素并改变植物激素代谢。南巴拉研究小组研究植物如何控制植物激素水平的细胞浓度，重点关注脱落酸（ABA）代谢。我们使用拟南芥种子发芽作为模型系统，因为种子有复杂的机制来感知环境条件。成熟、干燥的种子含有在适当的环境中恢复植物生命周期所需的所有成分。种子通过感知水分、养分、温度、成熟后和光照来确定是否存在合适的发芽环境，这些外部信号的变化会改变植物的激素代谢和信号，从而决定种子是发芽还是休眠。本研究旨在阐明种子感知环境信号和调节植物激素代谢的分子机制，重点研究硝酸盐对ABA分解代谢和种子萌发的调控作用。