Time Resolved EPR Studies of Natural and Artificial Photosynthesis

自然和人工光合作用的时间分辨 EPR 研究

• 批准号: RGPIN-2015-04021
• 负责人: vanderEst, Arthur
• 金额: $ 2.04万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=612723
• 关键词: Time; Resolved; EPR; Studies; Natural

The goal of the proposed research is to gain a fundamental understanding of the factors governing the efficiency of solar energy conversion in natural and artificial photosynthetic systems. Our suite of modern time resolved electron paramagnetic resonance (EPR) instruments allows us to measure the electron spin polarization associated with the energy conversion and to use it to study the initial steps of this process in detail. Our close collaborations with groups in the US, Germany, Russia and Japan give us access to a wide range of complementary spectroscopic, theoretical, biochemical and molecular biology techniques. Using this broad array of methods, we are putting together an exquisitely detailed picture of the energetics, kinetics and dynamics of these vitally important systems. We are now using this knowledge to aid in the design and characterization of artificial mimics of the natural systems. The studies of natural photosynthetic proteins focus on two main areas: (i) Investigations of the pathway and energetics of light-induced electron transfer (ii) Determination of the structure of the complexes in oxygen sensitive organisms. In the area of artificial photosynthesis we are developing two main types of systems: (i) Light-driven, photochemical cells, which are able to split water. (ii) Biohybrid complexes in which natural proteins are chemically modified to create photosystem mimics With our unique combination of biochemical and spectroscopic techniques and expertise in spin chemistry we expect to make significant progress in resolving these issues, which are critical to a full understanding of the efficiency of photochemical energy conversion.

拟议研究的目标是从根本上了解自然和人工光合系统中太阳能转换效率的影响因素。我们的现代时间分辨电子顺磁共振（EPR）仪器套件使我们能够测量与能量转换相关的电子自旋极化，并使用它来详细研究这一过程的初始步骤。我们与美国、德国、俄罗斯和日本的团队密切合作，使我们能够获得广泛的互补光谱、理论、生物化学和分子生物学技术。使用这些广泛的方法，我们正在整理这些至关重要的系统的能量学，动力学和动力学的精美详细的图片。我们现在正在利用这些知识来帮助设计和表征自然系统的人工模拟。 天然光合蛋白的研究主要集中在两个方面： (i)光致电子转移的途径和能量学研究 (ii)氧敏感生物中配合物结构的测定。 在人工光合作用领域，我们正在开发两种主要类型的系统： (i)光驱动的光化学细胞，能够分解水。 (ii)生物杂交复合物，其中天然蛋白质被化学修饰以产生光系统模拟物 凭借我们独特的生物化学和光谱技术以及自旋化学专业知识，我们预计将在解决这些问题方面取得重大进展，这些问题对于充分了解光化学能量转换的效率至关重要。