Molecular characterization of endothelial transcytosis by different tissue beds

不同组织床内皮转胞吞作用的分子特征

• 批准号: RGPIN-2015-05802
• 负责人: Lee, Warren
• 金额: $ 2.19万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=613756
• 关键词: Molecular; characterization; endothelial; transcytosis; different

Every blood vessel in the body is lined by a specialized layer of cells called endothelium. One of the critical functions of the endothelium is to prevent blood vessels from leaking and thereby losing blood, cells and proteins. The endothelial lining can become leaky when gaps form between the cells; alternatively, leak can occur through the body of each cell in the endothelial layer. This latter process, called transcytosis, occurs when an individual cell shuttles proteins and other molecules out of the blood and deposits them on the other side. Very little is known about transcytosis because it has been historically been very difficult to study. My lab has devised novel methods for detecting and measuring transcytosis by the endothelium. We will use these novel techniques to figure out how transcytosis is controlled by blood vessels in different parts of the body. This research is important as it will help us understand how blood vessels regulate their leakiness. It will be of great interest to biologists and physiologists in Canada and internationally. We anticipate that our work will set the stage for breakthroughs in our understanding of transcytosis, an important physiologic process in blood vessels that has been largely neglected up to now.

人体内的每一条血管都由一层称为内皮的特殊细胞构成。内皮的关键功能之一是防止血管渗漏，从而丢失血液，细胞和蛋白质。当细胞之间形成间隙时，内皮衬里可以变得渗漏;或者，渗漏可以通过内皮层中的每个细胞的主体发生。后一个过程称为转胞吞作用，发生在单个细胞将蛋白质和其他分子从血液中运送出来并将它们沉积在另一侧时。关于转胞吞作用知之甚少，因为它在历史上一直很难研究。我的实验室设计了新的方法来检测和测量内皮细胞的转胞吞作用。我们将使用这些新技术来弄清楚转胞吞是如何由身体不同部位的血管控制的。这项研究很重要，因为它将帮助我们了解血管如何调节它们的泄漏。它将引起加拿大和国际生物学家和生理学家的极大兴趣。我们预计，我们的工作将为我们对转胞吞作用的理解取得突破奠定基础，转胞吞作用是血管中的一个重要生理过程，迄今为止在很大程度上被忽视了。