Molecular characterization of endothelial transcytosis by different tissue beds

不同组织床内皮转胞吞作用的分子特征

• 批准号: RGPIN-2015-05802
• 负责人: Lee, Warren
• 金额: $ 2.19万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657076
• 关键词: Molecular; characterization; endothelial; transcytosis; different

******Every blood vessel in the body is lined by a specialized layer of cells called endothelium. One of the critical functions of the endothelium is to prevent blood vessels from leaking and thereby losing blood, cells and proteins. The endothelial lining can become leaky when gaps form between the cells; alternatively, leak can occur through the body of each cell in the endothelial layer. This latter process, called transcytosis, occurs when an individual cell shuttles proteins and other molecules out of the blood and deposits them on the other side. Very little is known about transcytosis because it has been historically been very difficult to study. My lab has devised novel methods for detecting and measuring transcytosis by the endothelium. We will use these novel techniques to figure out how transcytosis is controlled by blood vessels in different parts of the body. This research is important as it will help us understand how blood vessels regulate their leakiness. It will be of great interest to biologists and physiologists in Canada and internationally. We anticipate that our work will set the stage for breakthroughs in our understanding of transcytosis, an important physiologic process in blood vessels that has been largely neglected up to now.**

*人体内的每一条血管都有一层特殊的细胞，称为内皮细胞。内皮的关键功能之一是防止血管渗漏，从而导致血液、细胞和蛋白质的流失。当细胞之间形成间隙时，内皮细胞衬里可能会渗漏；或者，可能会通过内皮层中每个细胞的身体发生渗漏。后一种过程被称为跨细胞作用，当单个细胞将蛋白质和其他分子从血液中穿梭出来并将它们存放在血液的另一边时，就会发生这种情况。人们对跨细胞作用知之甚少，因为从历史上看，对它的研究非常困难。我的实验室设计了新的方法来检测和测量内皮细胞的跨细胞作用。我们将使用这些新技术来弄清楚人体不同部位的血管是如何控制跨细胞作用的。这项研究很重要，因为它将帮助我们了解血管是如何调节其渗漏的。这将引起加拿大和国际上生物学家和生理学家的极大兴趣。我们预计，我们的工作将为我们在理解跨细胞作用方面取得突破奠定基础，这是血管中的一个重要的生理过程，到目前为止，它在很大程度上被忽视了。