Zebrafish Advanced Life Support System

斑马鱼高级生命支持系统

• 批准号: RTI-2017-00600
• 负责人: VanRaay, Terence
• 金额: $ 10.92万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616721
• 关键词: Zebrafish; Advanced; Life; Support; System

This application is to replace and expand our current antiquated zebrafish holding units with modern life support systems that will enable us to take full advantage of the emerging genome editing tools. With the advent of such tools and the utility of the zebrafish model, many more researchers are embracing zebrafish in their research programs. Unfortunately, our current antiquated system is over capacity preventing the addition of new lines, ultimately inhibiting our research programs. More critically, our current system is over 26 years old with no monitors of water quality, parts no longer available and prone to failure putting our research at risk. Even with the best maintenance, increased wear and tear continues to degrade our current system. At the University of Guelph, several NSERC-funded research programs have or are currently developing numerous CRISPR-edited zebrafish lines, with more programs (representing 7 NSERC Discovery grants and 1 NSERC CRD grant application) requiring future genome-edited zebrafish. These programs research signal transduction pathways, developmental pathophysiology, fundamental contractile systems, reproductive biology, and aquatic environmental toxicology to grow fundamental discovery for the benefit of Canada’s environment and quality of life. However, to support the researchers at Guelph and take full advantage of modern techniques in zebrafish, there is an urgent need to upgrade and expand our current zebrafish facility. This proposal is for an advanced zebrafish rack and life support system that contains one central filtration and life support system. Importantly, an advanced monitoring and dosing system will maintain a reliable and consistent environment, which will ultimately reduce the number of fish required for experimentation. This modern system will expand our current antiquated system by >3-fold and provide us with the stability and confidence that we and our collaborators require after investing significant effort into making CRISPR mutant and transgenic zebrafish lines. With this equipment, a breadth of NSERC-funded biology research will be directly impacted while a new generation of researchers will be versed in discovery using the latest genome-editing technology, holding more promise than any other in the past century.

该应用程序旨在用现代生命支持系统替换和扩展我们当前过时的斑马鱼保存单元，这将使我们能够充分利用新兴的基因组编辑工具。随着此类工具的出现和斑马鱼模型的实用性，越来越多的研究人员在他们的研究项目中采用斑马鱼。不幸的是，我们当前过时的系统容量过剩，无法添加新线路，最终阻碍了我们的研究计划。更关键的是，我们当前的系统已经使用了 26 多年，没有水质监测器，零件不再可用，而且容易出现故障，使我们的研究面临风险。即使进行了最好的维护，磨损的增加也会继续降低我们当前系统的性能。 在圭尔夫大学，多个 NSERC 资助的研究项目已经或正在开发大量 CRISPR 编辑的斑马鱼品系，还有更多项目（代表 7 个 NSERC 发现资助和 1 个 NSERC CRD 资助申请）需要未来的基因组编辑斑马鱼。这些项目研究信号转导途径、发育病理生理学、基本收缩系统、生殖生物学和水生环境毒理学，以促进基础发现，造福加拿大的环境和生活质量。 然而，为了支持圭尔夫的研究人员并充分利用斑马鱼的现代技术，迫切需要升级和扩大我们现有的斑马鱼设施。该提案涉及一种先进的斑马鱼架和生命支持系统，其中包含一个中央过滤和生命支持系统。重要的是，先进的监测和剂量系统将维持可靠且一致的环境，这最终将减少实验所需的鱼类数量。这个现代系统将把我们当前过时的系统扩展超过 3 倍，并为我们和我们的合作者在投入大量精力制作 CRISPR 突变体和转基因斑马鱼系后提供所需的稳定性和信心。有了这些设备，NSERC 资助的广泛生物学研究将受到直接影响，而新一代研究人员将精通使用最新基因组编辑技术的发现，比上个世纪的任何其他研究人员都更有希望。