Mapping cell metabolism in tissues: NADPH/NADP+ redox state in the regulation of cell dedifferentiation, proliferation, and survival.

新陈代谢对细胞的分化、存活和增殖起着至关重要的作用。为了充分了解细胞代谢和这些不同反应之间的联系，我们需要高时间和空间分辨率的方法来测量代谢流量。罗切洛实验室长期以来一直对结合定量荧光显微镜和微流控设备(“芯片上的胰岛”)来测量活的胰岛中单个β细胞的新陈代谢感兴趣。胰岛是营养刺激的胰岛素分泌的一个有价值的模型系统，但也提供了研究组织内原代细胞的可塑性和生存的机会。我们之前的NSERC资金使我们能够开发一种光谱可调的遗传编码传感器(Apollo-NADP

Metabolism critically governs cell differentiation, survival, and proliferation. To fully understand the links between cell metabolism and these varied responses, we need methods that measure metabolic flux with high temporal and spatial resolution. The Rocheleau Lab has a long-standing interest in combining quantitative fluorescence microscopy and microfluidic devices ("islet-on-a-chip") to measure the metabolism of individual beta-cells within living pancreatic islets. Pancreatic islets are a valuable model system of nutrient-stimulated insulin secretion; yet also offer the opportunity to study the plasticity and survival of primary cells within a tissue. Our previous NSERC funding allowed us to develop a spectrally tunable genetically encoded sensor (Apollo-NADP