Mapping cell metabolism in tissues: NADPH/NADP+ redox state in the regulation of cell dedifferentiation, proliferation, and survival.

绘制组织中的细胞代谢图：NADPH/NADP 氧化还原状态对细胞去分化、增殖和存活的调节。

• 批准号: RGPIN-2016-06468
• 负责人: Rocheleau, Jonathan
• 金额: $ 2.77万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=628785
• 关键词: Mapping; cell; metabolism; tissues; NADPH

Metabolism critically governs cell differentiation, survival, and proliferation. To fully understand the links between cell metabolism and these varied responses, we need methods that measure metabolic flux with high temporal and spatial resolution. The Rocheleau Lab has a long-standing interest in combining quantitative fluorescence microscopy and microfluidic devices ("islet-on-a-chip") to measure the metabolism of individual beta-cells within living pancreatic islets. Pancreatic islets are a valuable model system of nutrient-stimulated insulin secretion; yet also offer the opportunity to study the plasticity and survival of primary cells within a tissue. Our previous NSERC funding allowed us to develop a spectrally tunable genetically encoded sensor (Apollo-NADP

代谢对细胞分化、存活和增殖起着至关重要的作用。为了充分了解细胞代谢与这些不同反应之间的联系，我们需要具有高时空分辨率的测量代谢通量的方法。Rocheleau实验室长期以来一直对结合定量荧光显微镜和微流体装置（“胰岛芯片”）来测量活胰岛内单个β细胞的代谢感兴趣。胰岛是营养刺激胰岛素分泌的一个有价值的模型系统；同时也为研究组织内原代细胞的可塑性和存活提供了机会。我们之前的NSERC资助让我们开发了一个光谱可调的基因编码传感器（阿波罗- nadp）