Design and fabrication of a standard to align multicolour TIRF microscopes.

设计和制造校准多色 TIRF 显微镜的标准。

• 批准号: 487070-2015
• 负责人: Rocheleau, Jonathan
• 金额: $ 1.82万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=585921
• 关键词: Design; fabrication; standard; align; multicolour

The proposed project is a collaborative effort between the Rocheleau lab and Spectral Applied Research Inc. ("Spectral"). Spectral is a biotechnology company based in Richmond Hill Ontario that aims to find innovative yet practical solutions to optical and opto-mechanical challenges, thereby producing outstanding value for the scientific, industrial and commercial market place (http://www.spectral.ca/home). They specialize in the research and development of custom optical instrumentation. Spectral requires a simple "in-the-field" method to quantify the performance of Total Internal Refection Fluorescence (TIRF) microscopes. More specifically, they need a standard slide to accurately measure the evanescent field penetration depth in multiple colours. They believe a slide with patterned nanoscale tall channels/features (i.e. channels filled with dye that are shorter than the evanescent field) could act as this standard slide; however, they lack the resources to invest directly in this approach. Dr. Rocheleau has unique expertise in microfluidic device design and quantitative fluorescence microscopy. This combined expertise will allow the Rocheleau lab in collaboration with Spectral staff scientists to fabricate and test the new standard slide. The Rocheleau lab has access to the resources needed to fabricate (Microfluidics Foundry) and test (TIRF microscopes) their designs. Spectral does not have access to these resources and it would be prohibitively expensive to develop them for such a short-term project. This proposal entails nanoimprinting microfluidic channels to pattern channels of varying height and thus provide easy standardization in the field.

拟议的项目是Rocheleau实验室和光谱应用研究公司之间的合作努力。（“Spectral”）。Spectral是一家位于安大略里士满山的生物技术公司，旨在为光学和光机械挑战找到创新而实用的解决方案，从而为科学，工业和商业市场创造卓越的价值（http：//www.spectral.ca/home）。他们专门从事定制光学仪器的研究和开发。光谱需要一个简单的“现场”方法来量化全内反射荧光（TIRF）显微镜的性能。更具体地说，他们需要一个标准的幻灯片，以准确地测量多种颜色的倏逝场穿透深度。他们认为，具有图案化纳米级高通道/特征（即，填充有比倏逝场短的染料的通道）的载玻片可以充当这种标准载玻片;然而，他们缺乏投资的资源 直接用这种方法。Rocheleau博士在微流控装置设计和定量荧光显微镜方面拥有独特的专业知识。这种结合的专业知识将使Rocheleau实验室与Spectral的科学家合作，制造和测试新的标准载玻片。Rocheleau实验室可以获得制造（Microfluidics Foundry）和测试（TIRF显微镜）所需的资源。Spectral无法获得这些资源，而且为这样一个短期项目开发这些资源的成本高得令人望而却步。该提议需要纳米压印微流体通道以图案化不同高度的通道，从而在该领域提供容易的标准化。