Characterization of novel Programmed Cell death (PCD) pathways in yeast

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Understanding Programmed Cell Death (PCD) is complicated by the existence of many different sub-forms that exists within and between the different types of PCD. The yeast S. cerevisiae undergoes a form of intrinsic apoptosis comparable to that seen in metazoans. By screening a mammalian expression cDNA library in yeast undergoing PCD, we identified a number of pro-survival sequences and we have now characterized 10 of these. We have made some interesting observations including the fact that human 14-3-3β/α prevents apoptotic like PCD as well as preventing PCD in response to prolonged autophagy. Presently, we are characterizing the anti-PCD function of human H-ferritin as well as a putative yeast ferritin encoded by the orphan gene RGI1. Within this context we propose the following 3 aims: