Functional Mapping of ABC Transporters Protein Interaction Networks

ABC 转运蛋白相互作用网络的功能图谱

• 批准号: RGPIN-2017-05009
• 负责人: Georges, Elias
• 金额: $ 1.89万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629266
• 关键词: Functional; Mapping; ABC; Transporters; Protein

The human genome encodes for 48 ABC proteins that are classified into seven subfamilies (ABC A - G). Research from several laboratories has shown these ABC proteins to transport various endogenous substrates, including chloride ions, glutathione, cholesterol, LTC4, hemin, sterols and bile salts; however little is known about the mechanisms that regulate their functions. We believe that such regulatory mechanisms can be unmasked by identifying ABC transporters’ interacting proteins. Using peptide-scanning approach to identify interacting proteins, we have shown that sequences in ABCB1 and ABCC1

人类基因组编码48种ABC蛋白，分为7个亚家族(ABC A-G)。几个实验室的研究表明，这些ABC蛋白可以运输各种内源性底物，包括氯离子、谷胱甘肽、胆固醇、LTC4、氯化血红素、甾醇和胆盐；然而，人们对它们的功能调节机制知之甚少。我们认为，通过鉴定ABC转运蛋白的相互作用蛋白，可以揭示这种调控机制。使用肽扫描方法鉴定相互作用的蛋白质，我们已经证明了ABCB1和ABCC1中的序列