Structural Engineering Principles of Centrosome Assembly by Super Resolution Microscopy Imaging

超分辨率显微镜成像中心体组装的结构工程原理

• 批准号: RGPIN-2015-04795
• 负责人: Mennella, Vito
• 金额: $ 2.55万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629460
• 关键词: Structural; Engineering; Principles; Centrosome; Assembly

My research program is aimed at determining the engineering principles of cell function through the analysis of its macromolecular assemblies. I focused my attention on the centrosome, an organelle critical for human development, which organizes the microtubule cytoskeleton. Centrosomes are composed of two large structures: the centriole, a 9-fold symmetric cylindrical structure encircled by microtubule blades, and the pericentriolar material, traditionally described and understood as an amorphous, electron-dense material that surrounds the centrioles. The pericentriolar material was discovered over 100 years ago, but there had been no significant advances in understanding its organization. This lack of knowledge is in part due to the shortage of effective methods to study the structural organization of macromolecular assemblies within the cell. I recently designed, in collaboration with other scientists, a super-resolution imaging and analysis strategy capable of determining the position of proteins with nanometer precision.

我的研究项目旨在通过分析细胞的大分子组合来确定细胞功能的工程原理。我把注意力集中在中心体上，这是一种对人类发育至关重要的细胞器，负责组织微管细胞骨架。中心体由两个大结构组成：中心粒，一个由微管叶片包围的9倍对称圆柱形结构，以及中心粒周围物质，传统上被描述和理解为包围中心粒的无定形、电子密集的物质。心泡周围物质是在100多年前发现的，但在了解其组织方面没有取得重大进展。这种知识的缺乏部分是由于缺乏有效的方法来研究细胞内大分子组装的结构组织。我最近与其他科学家合作设计了一种超分辨率成像和分析策略，能够以纳米精度确定蛋白质的位置。