Molecular mechanisms and applications of monomeric DNA endonucleases

单体DNA核酸内切酶的分子机制及应用

• 批准号: RGPIN-2015-04800
• 负责人: Edgell, David
• 金额: $ 3.79万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629829
• 关键词: Molecular; mechanisms; applications; monomeric; DNA

My program of research deals with mobile genetic elements, discrete units of DNA that promote their own spread within and between genomes. For the past ten years, my laboratory has studied the evolution and function of homing endonucleases, site-specific DNA endonucleases that spread by making a double-strand break (or nick) in a DNA target. Over the last funding period, we focused on basic mechanistic questions concerning DNA cleavage by the GIY-YIG family of homing endonucleases, and translating this mechanistic understanding to the generation of novel monomeric chimeric nucleases for genome-editing applications. Academic and industrial researchers use genome-editing reagents to introduce precise modifications in complex genomes for applications in agriculture, aquaculture, synthetic biology, and discovery-based research in model organisms.

我的研究项目涉及移动遗传元件，即促进其在基因组内部和基因组之间传播的离散 DNA 单元。在过去的十年里，我的实验室研究了归巢核酸内切酶的进化和功能，这些酶是通过在 DNA 靶标中产生双链断裂（或切口）来传播的位点特异性 DNA 核酸内切酶。在上一个资助期间，我们专注于有关归巢核酸内切酶 GIY-YIG 家族切割 DNA 的基本机制问题，并将这种机制理解转化为用于基因组编辑应用的新型单体嵌合核酸酶的生成。学术和工业研究人员使用基因组编辑试剂对复杂基因组进行精确修饰，以应用于农业、水产养殖、合成生物学和模式生物的基于发现的研究。