A DNA printer for gene synthesis and synthetic biology

用于基因合成和合成生物学的 DNA 打印机

• 批准号: RTI-2018-00315
• 负责人: Edgell, David
• 金额: $ 7.26万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642155
• 关键词: DNA; printer; gene; synthesis; synthetic

This NSERC RTI application is requesting funds to purchase a bench-top DNA synthesizer to “print” long DNA fragments for synthetic biology applications. Synthetic biology can be thought of as molecular lego, where segments of DNA with defined functions are assembled to build larger and larger “machines” with defined functions. Some examples of synthetic biology applications are building genetic pathways for production of natural products, to build artificial plasmids or chromosomes, and to build genetic switches to control gene expression. Underlying all of synthetic biology is the requirement to rapidly and accurately synthesize long DNA fragments. The requested equipment represents the state-of-the-art in benchtop DNA synthesis, allowing allow researchers to “print” up to 32 DNA fragments at a time. The requested DNA printer is more cost and time effective than using commercial sources. The DNA printer will enable the research projects of a large group of researchers at Western University, and allow our research trainees unparalleled access to the latest synthetic biology technology, positioning them for future success.

这个NSERC RTI申请正在申请资金购买一台台式DNA合成仪，用于合成生物学应用的长DNA片段的“打印”。合成生物学可以被认为是分子乐高积木，其中具有定义功能的DNA片段被组装成具有定义功能的越来越大的“机器”。合成生物学应用的一些例子是构建用于生产天然产物的遗传途径，构建人工质粒或染色体，以及构建控制基因表达的遗传开关。所有合成生物学的基础是快速准确地合成长DNA片段的要求。所要求的设备代表了台式DNA合成的最新技术水平，允许研究人员一次“打印”多达32个DNA片段。所要求的DNA打印机比使用商业来源更具成本效益和时间效益。DNA打印机将使西方大学的一大群研究人员的研究项目，并允许我们的研究学员无与伦比的访问最新的合成生物学技术，定位他们为未来的成功。