Role of chromatin structure and histone modifications in the regulation of DNA repair by homologous recombination

染色质结构和组蛋白修饰在同源重组 DNA 修复调节中的作用

• 批准号: 435636-2013
• 负责人: Wurtele, Hugo
• 金额: $ 2.55万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629839
• 关键词: Role; chromatin; structure; histone; modifications

DNA damage can lead to the inactivation or misregulation of important genes and cause metabolic malfunction or cell death. Fortunately, cells possess DNA repair mechanisms that allow them to survive in the presence of DNA lesions. In order to fit in the tiny confines of the cell, DNA is packaged into a structure called chromatin. We are investigating how chromatin structure participates in the response to DNA damage. We propose to use yeast as a model organism to understand how variants of histone proteins, which are basic building blocks of chromatin, can influence DNA repair. We anticipate that a better understanding of these fundamental processes will unearth novel links between specific determinants of chromatin structure and the response to environmental DNA damaging agents. This research will thus improve our comprehension of how cells maintain the integrity of their genomes.

DNA损伤可导致重要基因的失活或失调，并导致代谢功能障碍或细胞死亡。幸运的是，细胞具有DNA修复机制，使它们能够在DNA损伤的情况下存活。为了适应细胞的微小范围，DNA被包装成一种称为染色质的结构。我们正在研究染色质结构如何参与对DNA损伤的反应。我们建议使用酵母作为模式生物，以了解组蛋白的变体，这是染色质的基本组成部分，可以影响DNA修复。我们预计，更好地了解这些基本过程将发掘染色质结构的特定决定因素和对环境DNA损伤剂的反应之间的新联系。因此，这项研究将提高我们对细胞如何保持其基因组完整性的理解。