Molecular characterization of the function and regulation of the subcomplexes of the ribosomal small subunit processome in ribosome assembly in the yeast model organism Saccharomyces cerevisiae

酵母模型生物酿酒酵母核糖体组装中核糖体小亚基加工组亚复合体的功能和调节的分子表征

• 批准号: RGPIN-2016-06599
• 负责人: Charette, JMichael
• 金额: $ 2.26万
• 依托单位: Brandon University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629909
• 关键词: Molecular; characterization; function; regulation; subcomplexes

Ribosomes are tiny molecular machines that are present by the hundreds of thousands in all cells. They play an essential role in every aspect of life, as they are the link between the genome (or DNA) and proteins. Ribosomes “read” genetic information and translate it into the corresponding protein. In turn, proteins provide the cell with architectural and structural support, the ability to move (such as muscle contraction), and often themselves act as miniature molecular motors in providing the cell with the ability to use energy, to grow, and to divide. These are all key aspects to the normal day-to-day life of a cell. We have an increasingly sophisticated understanding of how ribosomes read genetic material and assemble the corresponding protein, much like we understand well the internal combustion motor of a car. The research proposed here looks at how the ribosome is assembled and at another miniature molecular machine, called the SSU processome, which assembles part of the ribosome. That is akin to my saying that my interests are in how cars are assembled and how car assembly lines work.

核糖体是微小的分子机器，在所有细胞中存在数十万个。它们在生命的各个方面都发挥着重要作用，因为它们是基因组（或DNA）和蛋白质之间的联系。核糖体“读取”遗传信息并将其翻译成相应的蛋白质。反过来，蛋白质为细胞提供建筑和结构支持，运动（如肌肉收缩）的能力，并且通常本身充当微型分子马达，为细胞提供使用能量，生长和分裂的能力。这些都是细胞正常日常生活的关键方面。我们对核糖体如何读取遗传物质并组装相应蛋白质的理解越来越复杂，就像我们对汽车内燃机的理解一样。这里提出的研究着眼于核糖体是如何组装的，以及另一种微型分子机器，称为SSU加工体，它组装核糖体的一部分。这类似于我说我的兴趣是汽车如何组装和汽车装配线如何工作。