Conformational Switching and Energetics in the Maltose Transporter

麦芽糖转运蛋白的构象转换和能量学

• 批准号: 217494-2013
• 负责人: Shilton, Brian
• 金额: $ 3.64万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630043
• 关键词: Conformational; Switching; Energetics; Maltose; Transporter

Living cells are delimited by membranes that restrict the movement of molecules between the cell's cytoplasm and the external environment. Cells must grow, divide, and communicate with each other, and to accomplish these things, molecules must be transported across the membrane. Membrane transporters are protein machines that are embedded in the membrane and use energy to move specific molecules into or out of the cell. The proposed research is focussed on understanding the function of a group of transport systems that bacteria use to acquire nutrients from their environment. The nutrient acquisition systems in bacteria, also known as "ATP Binding Cassette (ABC) Import Systems", function as a kind of "vacuum cleaner", picking up nutrients from the environment and moving them into the cell. The systems have a peripheral protein on the outside of the cell that binds to a nutrient molecule and then interacts with membrane-embedded components. The interaction between the nutrient-bound peripheral protein and the membrane-embedded components causes changes in the structures of both partners, leading to hydrolysis of ATP (the cell's energy "currency") along with movement of the nutrient through the membrane-embedded components and into the cytoplasm.The proposed studies will define the individual molecular events that occur during the transport process, and help us to understand how energy is coupled to acquisition of nutrients. The research may offer routes to new antibiotics, and the knowledge gained can be applied to related ABC transport systems that have important functions in all forms of life.

活细胞由限制细胞质和外部环境之间分子运动的膜界定。细胞必须生长，分裂，并相互交流，为了完成这些事情，分子必须穿过膜。膜转运蛋白是嵌入在膜中的蛋白质机器，并使用能量将特定分子移入或移出细胞。这项研究的重点是了解细菌从环境中获取营养物质的一组运输系统的功能。细菌中的营养获取系统，也被称为“ATP结合盒（ABC）输入系统”，其功能就像一种“真空吸尘器”，从环境中拾取营养物质并将其移动到细胞中。该系统在细胞外部有一种外周蛋白，它与营养分子结合，然后与膜嵌入的成分相互作用。营养结合的外周蛋白质和膜包埋组分之间的相互作用引起双方结构的变化，导致ATP水解（细胞的能量“货币”）沿着营养物质通过膜包埋成分进入细胞质的运动。拟议的研究将定义运输过程中发生的单个分子事件，帮助我们理解能量是如何与营养物质的获取相结合的。这项研究可能会提供新抗生素的途径，所获得的知识可以应用于在所有生命形式中具有重要功能的相关ABC运输系统。