Biochemical, proteomic and bioinformatic insights into Arabidopsis thaliana protein phosphatases

对拟南芥蛋白磷酸酶的生物化学、蛋白质组学和生物信息学见解

• 批准号: 216895-2013
• 负责人: Moorhead, Gregory
• 金额: $ 4.15万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630289
• 关键词: Biochemical; proteomic; bioinformatic; insights; into

Plants, like all other organisms, use proteins to do the majority of specific jobs inside cells. These jobs or roles can be structural, transport, storage and enzymatic, among others. Once made, protein function or activity is often controlled by specific modifications such as the covalent addition of other functional moieties, like phosphoryl groups. The addition and removal of phosphoryl groups on proteins is a regulatory mechanism called protein phosphorylation. The enzymes that add and remove these phosphoryl groups are known as protein kinases and protein phosphatases. The focus of my research program is to elucidate the specific roles and regulation of the protein phosphatases. Protein phosphatases are enzymes or catalytic engines, that by association with other proteins gain specificity in their jobs. This proposal will use biochemical, molecular biology and proteomic methods, including mass spectrometry, to identify these protein phosphatase interacting proteins. We will also employ bioinformatic methods to identify the protein phosphatases in other organisms so that we can resolve the evolutionary history of these complex enzymes. These goals will be accomplished by using a model plant species called Arabidopsis thaliana.

像所有其他生物一样，植物使用蛋白质来完成细胞内的大部分特定工作。这些工作或角色可以是结构性的、运输的、仓储的和酶的等等。一旦形成，蛋白质的功能或活性通常由特定的修饰来控制，例如其他功能部分的共价加成，如磷酰基。蛋白质上磷酸基团的添加和去除是一种称为蛋白质磷酸化的调节机制。添加和移除这些磷酰基的酶称为蛋白激酶和蛋白磷酸酶。我的研究计划的重点是阐明蛋白磷酸酶的具体作用和调节。蛋白磷酸酶是一种酶或催化引擎，通过与其他蛋白质结合，在其工作中获得特异性。这项提案将使用生化、分子生物学和蛋白质组学方法，包括质谱学，来鉴定这些蛋白磷酸酶相互作用的蛋白质。我们还将利用生物信息学的方法来鉴定其他生物中的蛋白磷酸酶，以便我们能够解析这些复杂酶的进化历史。这些目标将通过使用一种名为拟南芥的模式植物物种来实现。