权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Replacement Light Scattering Instrument and Capability Enhancement – Measuring Biomolecular Size and Surface Charge

替代光散射仪器和能力增强 — 测量生物分子尺寸和表面电荷

基本信息

批准号：
RTI-2018-00281
负责人：
Graether, Steffen
金额：
$ 8.39万
依托单位：
University of Guelph
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments
财政年份：
2017
资助国家：
加拿大
起止时间：
2017-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642152
关键词：
Replacement Light Scattering Instrument Capability

项目摘要

We propose to purchase a nano-scale dynamic light scattering system (specifically a Malvern Zetasizer nano ZSP) to both replace our broken unit purchased in 2003 and to increase our capability to examine several properties of biomolecules in solution. Characterizing the size and molecular weight of biomolecules has applications in diverse fields as is reflected in the diverse research interests and expertise of the applicants. The Graether lab is interested in understanding how plant proteins, named dehydrins, are able to protect cell membranes from damage caused by cold and drought; the Zetasizer will be used to elucidate how dehydrins prevent membrane damage caused by membrane fusion. The Dutcher lab will use the unit to measure the size of phytoglycogen, a highly branched sugar polymer, as a carrier for bioactive molecules. The Brown and Ladizhansky labs will examine the preparation of membrane protein – membrane complexes, a necessary step before carrying out high-resolution structural studies. Beyond proteins and carbohydrates, the Zetasizer will be used by engineers to study the treatment of waste. The Pensini lab uses chemical engineering methods to treat waste at contaminated sites using switchable surfactants, while the Abbassi lab treats wastewater. The Zetasizer will be used to measure both the size and charge of samples to optimize the treatment and separation of these two kinds of waste.

我们建议购买一个纳米级动态光散射系统（特别是马尔文Zetasizer纳米ZSP），以取代我们在2003年购买的破碎单元，并提高我们的能力，检查溶液中的生物分子的几个属性。表征生物分子的大小和分子量在不同领域中具有应用，这反映在申请人的不同研究兴趣和专业知识中。Graether实验室有兴趣了解植物蛋白质，命名为tetrains，如何能够保护细胞膜免受寒冷和干旱造成的损害; Zetasizer将用于阐明tetrains如何防止膜融合造成的膜损伤。Dutcher实验室将使用该装置测量植物糖原的大小，植物糖原是一种高度分支的糖聚合物，作为生物活性分子的载体。Brown和Ladizhansky实验室将研究膜蛋白-膜复合物的制备，这是进行高分辨率结构研究之前的必要步骤。除了蛋白质和碳水化合物，Zetasizer还将被工程师用于研究废物处理。Pensini实验室使用化学工程方法，使用可切换的表面活性剂处理受污染场地的废物，而Abbassi实验室则处理废水。Zetasizer将用于测量样品的尺寸和电荷，以优化这两种废物的处理和分离。